Context: Inactivating mutations of PRKAR1A, the regulatory subunit type 1A (RI) of protein kinase A (PKA), are associated with tumor formation.

Objective: To evaluate the role of PKA isozymes on proliferation and cell cycle.

Methods: A cell line with RI haploinsufficiency due to an inactivating PRKAR1A mutation (IVS2+1 G>A) was transfected with constructs encoding PKA subunits. Genetics, PKA subunit mRNA and protein expression and proliferation, aneuploidy and cell cycle status were assessed. To identify factors that mediate PKA-associated cell cycle changes, we studied E2F and cyclins expression in trasfected cells and E2F's role by siRNA; we also assessed cAMP levels and baseline and stimulated cAMP signaling in transfected cells.

Results: Introduction of PKA subunits led to changes in proliferation and cell cycle: a decrease in aneuploidy and G2/M for the PRKAR1A-transfected cells and an increase in S phase and aneuploidy for cells transfected with PRKAR2B, a known PRKAR1A mutant (RIP), and the PKA catalytic subunit. There were alterations in cAMP levels, PKA subunit expression, cyclins and E2F factors; E2F1 was shown to possibly mediate PKA effects on cell cycle by siRNA studies. cAMP levels and constitutive and stimulated cAMP signaling were altered in transfected cells.

Conclusion: This is the first immortalized cell line with a naturally occurring PRKAR1A-inactivating mutation that is associated in vivo with tumor formation. PKA isozyme balance is critical for the control of cAMP signaling and related cell cycle and proliferation changes. Finally, E2F1 may be a factor that mediates dysregulated PKA's effects on the cell cycle.