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This version published online on November 6, 2007
Journal of Clinical Endocrinology & Metabolism, doi:10.1210/jc.2007-1313
A more recent version of this article appeared on January 1, 2008
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Submitted on June 13, 2007
Accepted on October 26, 2007

Catecholamine regulation of local lactate production in vivo in skeletal muscle and adipose tissue: role of {beta}–adrenoreceptor subtypes

Veronica Qvisth*, Eva Hagström-Toft, Staffan Enoksson, and Jan Bolinder

Dept of Medicine, Karolinska University Hospital - Huddinge, Karolinska Institutet, Stockholm, Sweden; Dept of Vascular Surgery, Karolinska University Hospital - Solna, Karolinska Institutet, Stockholm, Sweden

* To whom correspondence should be addressed. E-mail: veronica.qvisth{at}ki.se.

Context: The regulation of lactate production in skeletal muscle (SM) and adipose tissue (AT) is not fully elucidated.

Objective: To investigate the catecholamine-mediated regulation of lactate production and blood flow in SM and AT in healthy, normal – weight subjects by using microdialysis.

Methods: First, lactate levels in SM and in AT were measured during an intravenous norepinephrine infusion (n = 11). Local blood flow was determined with the 133Xe-clearance technique. Second, muscle lactate was measured during hypoglycaemia and endogenous epinephrine stimulation (n = 12). Third, SM was perfused with selective {beta}1-3–adrenoreceptor agonists in situ (n = 8). Local blood flow was measured with ethanol perfusion technique.

Results: In response to i.v. norepinephrine the fractional release of lactate (difference between tissue and arterial lactate) increased by 40% in SM (p=0.001), while remaining unchanged in AT. Blood flow decreased by 40% in SM (p<0.005) and increased by 50% in AT (p<0.05).

In response to hypoglycaemia, epinephrine increased 10-fold and the fractional release of lactate in SM doubled (p<0.0001). The blood flow remained unchanged.

The {beta}2-agonist, terbutaline caused a marked concentration-dependent increase of muscle lactate and blood flow (p<0.0001). The {beta}1-agonist, dobutamine induced a discrete increase of muscle lactate (p<0.0001) and the blood flow remained unchanged. The {beta}3-agonist CPG 12177 did not affect muscle lactate or blood flow.

Conclusion: Catecholamines stimulate lactate production in skeletal muscle but not in adipose tissue. In skeletal muscle, the {beta}2-adrenoreceptor is the most important beta-adrenergic receptor subtype in the regulation of lactate production.
Key words: microdialysis • lactate • adipose tissue • skeletal muscle • blood flow • {beta} -adrenoreceptors • norepinephrine






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