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Submitted on November 9, 2005
Accepted on March 29, 2006
Cattedra di Fisiopatologia della Riproduzione Umana, Universita' Cattolica del Sacro Cuore (UCSC), 00168 Roma, Italy; Istituto Scientifico Internazionale "Paolo VI", UCSC, 00168 Roma, Italy; Istituto di Ricerca "Associazione OASI Maria SS ONLUS", 94018 Troina (EN), Italy; Istituto di Farmacologia, UCSC, 00168 Roma, Italy
* To whom correspondence should be addressed. E-mail: krimisa{at}libero.it.
Context. Vascular Endothelial Growth Factor (VEGF) is essential for normal luteal development and function but little is still known about the regulation of its production by human mid-luteal phase luteal cells.
Objective. We investigated whether human chorionic gonadotropin (hCG) or local factors, including hypoxia (CoCl2-chemical hypoxia), Insulin-like Growth Factor (IGF)-I and IGF-II, prostaglandin (PG) E2, and PGF2
, prevail in modulating VEGF mRNA and protein production in human mid-luteal phase luteal cells. The effect of progesterone (P) on luteal VEGF mRNA expression and protein secretion was also evaluated. Finally, we investigated whether VEGF could directly affect luteal P secretion.
Interventions. In human mid-luteal phase luteal cells VEGF mRNA expression was evaluated by semiquantitative RT-PCR, whereas VEGF and P release by ELISA and RIA, respectively.
Results. hCG was unable to significantly affect luteal VEGF mRNA and protein synthesis, that in turn was significantly increased by both CoCl2 and IGFs. Conversely, VEGF mRNA and protein production was reduced by PGs and P. Finally, VEGF did not affect P luteal secretion.
Conclusions. Our results suggest that local ovarian factors, rather than hCG, predominate in regulating VEGF mRNA and protein production by human mid-luteal phase luteal cells. For VEGF a lack of a direct luteal steroidogenic effect was also demonstrated.
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