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Submitted on February 9, 2004
Accepted on November 23, 2004
3-adrenoceptor is the predominant -adrenoceptor subtype in human myometrium and its expression is up regulated in pregnancy
UPRES EA220 - Pharmacology, UFR Biomédicale des Saints Pères, 45 rue des Saints Pères 75006 Paris France; INSERM U427 and Department of Gynecology, Port-Royal Maternity Hospital, 123 Bd de Port Royal, 75014 Paris France; Department of Gynecology, CHU du Bocage, 21 Bd de Lattre de Tassigny, BP 1542, 21000 Dijon France; Sanofi-Aventis Research Center, Via Piranesi, 38. 20137 Milan Italy; Laboratory of Cardiovascular Physiopathology and Pharmacology, Faculty of Medicine, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon France
* To whom correspondence should be addressed. E-mail: celine.rouget{at}univ-paris5.fr.
In order to assess whether pregnancy might influence the functionality and the expression of human myometrial 
2- and 3-adrenoceptors (2- and 3-AR), we performed functional, binding, Western blot and molecular biology experiments in human non-pregnant and near-term pregnant myometrium. Inhibition of spontaneous contractions induced by a 3-AR agonist, SR 59119A, was significantly greater in pregnant compared with non-pregnant myometrial strips (E'max = 61 ± 5% vs. 44 ± 5% for pregnant and non-pregnant myometrium respectively) whereas salbutamol, a 2-AR agonist, was significantly less efficient in pregnant compared with non-pregnant myometrium (Emax = 29 ± 4% vs. 54 ± 8%). Although two populations of binding sites corresponding to 2- and 3-AR were identified in both non-pregnant and pregnant myometrium, we found a clear predominance of the 3-AR subtype. Moreover, 3-AR binding sites were up-regulated 2-fold in myometrium at the end of pregnancy. Both 2- and 3-AR mRNA were expressed in human non-pregnant and pregnant myometrium. Contrary to 2-AR, the expression of the 3-AR transcripts and immunoreactive proteins were increased in pregnant compared with non-pregnant myometrium. Such compelling data suggest a predominant role for 3-AR in the regulation of human myometrium contractility, especially at the end of pregnancy, which might have important consequences for the clinical management of preterm labor.
2- and 3-adrenoceptors •
pregnancy •
human myometrium •
mRNA •
binding experiments •
western blotting analysis
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