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Erratum

In the article "Adenosine 5'-Triphosphate Activates Nuclear Translocation of Mitogen-Activated Protein Kinases Leading to the Induction of Early Growth Response 1 and Raf Expression in Human Granulosa-Luteal Cells" by Chen-Jei Tai, Shu-Ju Chang, Peter C. K. Leung, and Chii-Ruey Tzeng (The Journal of Clinical Endocrinology & Metabolism 89:5189–5195, 2004), Figs. 1 and 3 are incorrect. The correct figures and their respective legends follow.

View larger version (40K): [in this window] [in a new window]   FIG. 1. The effect of ATP on MAPK activation in hGLCs using Western blot analysis. A, The dose-response of ATP on ERK1/2 (p44/p42) activation. hGLCs were treated with increasing concentrations of ATP (0, 100 nM, 1 µM, 10 µM, or 100 µM) for 5 min, as described in Materials and Methods. B, The time effect of ATP on ERK1/2 (p44/p42) activation. hGLCs were treated with 10 µM ATP for 0, 1, 5, 10, or 20 min, as described in Materials and Methods. C, The dose-response of ATP on p38 activation in hGLCs. D, The time effect of ATP on p38 activation in hGLCs. E, The dose-response of ATP on JNK activation in hGLCs. F, The time effect of ATP on JNK activation in hGLCs. The amount of loading was normalized by total ERK. The data are shown as relative ratio to basal levels. Values are presented as the mean ± SE of three individual experiments. Statistical analysis was performed by one-way ANOVA followed by the Tukey test. *, Differences were considered significant at P < 0.05.

View larger version (77K): [in this window] [in a new window]   FIG. 2. Biotinylated cDNA probes were synthesized from 5 µg total RNA of hGLCs in the absence (A) or presence (B) of 10 µM ATP and hybridized to the GEArray membrane spotted with 23 gene-specific cDNA fragments. After the hybridization, the membrane was incubated with alkaline phosphatase-conjugated streptavidin, and the signal was visualized with CDPStar, a chemiluminescent substrate, and exposed to x-ray film. C, Biotinylated cDNA probes were synthesized from 5 µg total RNA of hGLCs pretreated with MEK inhibitor (PD98059) before 10 µM ATP exposure (2E and 2F, egr-1; 8A and 8B, c-raf-1; 8E and 8F, GAPDH).

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