help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rao, Ch. V.
Right arrow Articles by D., Ph.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Rao, Ch. V.
Right arrow Articles by D., Ph.
The Journal of Clinical Endocrinology & Metabolism Vol. 82, No. 8 2756-2757
Copyright © 1997 by The Endocrine Society

Letters to the Editor

Immunocytochemical Localization of Gonadotropin and Gonadal Steroid Receptors in Human Pineal Glandsd

Ch. V. Rao and Ph. D.

University of Louisville Health Sciences Center Louisville, KY 40292

Our laboratory pioneered in demonstrating the presence of functional LH/hCG receptors in brain and in neuroendocrine cells (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11). We did not examine the pineal glands. Thus, seeing an article by Luboshitzky et al. in The Journal of Clinical Endocrinology and Metabolism 82:977–981, was very interesting to us.

The article described the localization of LH and FSH receptors in human pineal glands using the antibodies to receptors for LH (clone ZSL11) and FSH (clone ZMFS1), purchased from Zymed Laboratories. According to the company’s catalog, these antibodies are not for receptors. Instead they are made against the corresponding hormones. Thus, the authors have localized LH and FSH, not their receptors in human pineal glands, and the conclusions in the paper should be corrected. Detection of receptors through the use of antibodies to ligands is possible, but this is not what these authors have done.

Footnotes

d Received April 9, 1997. Address correspondence to: Ch. V. Rao, Ph.D., Professor and Director, Laboratory of Molecular Reproductive Biology and Medicine, University of Louisville Health Sciences Center, Louisville, Kentucky 40292.

References

  1. Lei ZM, Rao Ch V, Kornyei JL, Licht P, Hiatt ES. 1993 Novel expression of human chorionic gonadotropin/luteinizing hormone receptor gene in brain. Endocrinology. 132:2262–2270.[Abstract]
  2. Lei ZM, Rao Ch V. 1994 Novel presence of luteinizing hormone/human chorionic gonadotropin (hCG) receptors and the down-regulating action of hCG on gonadotropin releasing hormone gene expression in immortalized hypothalamic GT1–7 neurons. Mol Endocrinol. 8:1111–1121.[Abstract]
  3. Toth P, Lukacs H, Hiatt ES, Reid KH, Iyer V, Rao Ch V. 1994 Administration of human chorionic gonadotropin affects sleep-wake phases and other associated behaviors in cycling female rats. Brain Res. 654:181–190.[CrossRef][Medline]
  4. Lukacs H, Hiatt ES, Lei ZM, Rao Ch V. 1995 Peripheral and intracerebroventricular administration of human chorionic gonadotropin alters several hippocampus associated behaviors in cycling female rats. Hormones and Behavior. 29:42–58.[CrossRef][Medline]
  5. Lei ZM, Rao Ch V. 1995 Signaling and transacting factors in the transcriptional inhibition of gonadotropin releasing hormone gene by human chorionic gonadotropin in immortalized hypothalamic GT1–7 neurons. Mol Cell Endocrinol. 109:151–157.[CrossRef][Medline]
  6. Huang ZH, Lei ZM, Rao Ch V. 1995 Immortalized anterior pituitary {alpha}T3 gonadotropes contain functional luteinizing hormone/human chorionic gonadotropin receptors. Mol Cell Endocrinol. 114:217–222.[Medline]
  7. Li X, Lei ZM, Rao Ch V. 1996 Human chorionic gonadotropin down-regulates the expression of gonadotropin releasing hormone receptor gene in GT1–7 neurons. Endocrinology. 137:899–904.[Abstract]
  8. AL-Hader AA, Lei ZM, Rao Ch V. 1997 Novel expression of functional luteinizing hormone/chorionic gonadotropin receptors in cultured glial cells from neonatal rat brains. Biol Reprod. 56:501–507.[Abstract]
  9. AL-Hader AA, Lei ZM, Rao Ch V. 1997 Neurons from fetal rat brains contain functional luteinizing hormone/chorionic gonadotropin receptors. Biol Reprod. 56:1071–1076.[Abstract]
  10. Lei ZM, Rao Ch V. 1997 Cis-acting elements and trans-acting proteins in the transcriptional inhibition of gonadotropin releasing hormone gene by human chorionic gonadotropin in immortalized hypothalamic GT1–7 neurons. J Biol Chem. 272:14365–14371.[Abstract/Free Full Text]
  11. Huang Z-H, Lei ZM, Rao Ch V. 1997 Transcriptional and posttranscriptional mechanisms in human chorionic gonadotropin and gonadotropin releasing hormone regulation of luteinizing hormone-{alpha} subunit gene in {alpha}T3 gonadotrope cells. Mol Cell Endocrinol. In press.




This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rao, Ch. V.
Right arrow Articles by D., Ph.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Rao, Ch. V.
Right arrow Articles by D., Ph.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals