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Early Diagnosis by Genetic Analysis of Differentiated Thyroid Cancer Metastases in Small Lymph Nodes

Cattedra di Endocrinologia, Dipartimento di Medicina Sperimentale e Clinica (F.A., D.R., N.P., S.F.), Cattedra di Farmacologia, Facoltà di Farmacia (D.R.), Università di Reggio Calabria, 88100 Catanzaro, Italy, Cattedra di Endocrinologia, Ospedale Garibaldi, Università di Catania, Italy (D.G., A.I., R.V.)

Address correspondence and requests for reprints to: Sebastiano Filetti, M.D., Cattedra di Endocrinologia, Dipartimento di Medicina Sperimentale e Clinica, Via T. Campanella, 88100 Catanzaro, Italy.

Abstract

We report a PCR-based technique for detecting thyroid cancer metastases in small nodes <1.5 cm diameter by the amplification of thyroid specific transcripts TSH-receptor and thyroglobulin. A 100% correspondence with the histopathological diagnosis was observed in the 41/46 nodes (89%) in which an adequate sample was obtained at fine needle aspiration. The genetic analysis that resulted was more sensitive and accurate than both the cytological analysis (28% inadequate samples, 17% false negative diagnoses) and the thyroglobulin measurement in the aspirates (39% false negatives). The PCR-based genetic analysis may provide a useful tool for diagnosis and follow-up of thyroid cancer.

Received February 3, 1997.

Revised March 6, 1997.

Accepted March 10, 1997.

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This Article Abstract Full Text (PDF) Submit a related Letter to the Editor Purchase Article View Shopping Cart Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arturi, F. Articles by Filetti, S. Search for Related Content PubMed PubMed Citation Articles by Arturi, F. Articles by Filetti, S.