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Human Glucocorticoid Receptor Gene (NR3C1) Pharmacogenomics: Gene Resequencing and Functional Genomics

Division of Clinical Pharmacology, Department of Molecular Pharmacology and Experimental Therapeutics (N.N., V.M., I.M., L.L.P., L.W.), Division of Biomedical Informatics, Department of Health Sciences Research (K.R.K.), Department of Biochemistry and Molecular Biology (B.W.E., E.D.W.), and Division of Biostatistics, Department of Health Sciences Research (D.J.S.), Mayo Clinic, Rochester, Minnesota 55905

Address all correspondence and requests for reprints to: Liewei Wang, M.D., Ph.D., Division of Clinical Pharmacology, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905. E-mail: wang.liewei{at}mayo.edu.

Context: The human glucocorticoid receptor (GR) is a nuclear hormone receptor that regulates multiple physiological and pathophysiological processes. There are large variations in both physiological and therapeutic response to glucocorticoids. Multiple previous studies suggested that genetic polymorphisms in GR (NR3C1) might play an important role.

Objective: The aim of the study was to identify and determine the functional implications of common genetic variation in NR3C1.

Design: We resequenced the NR3C1 gene using 240 DNA samples from four ethnic groups, followed by functional characterization of the effects of selected polymorphisms.

Results: A total of 108 polymorphisms were identified in GR, including nine nonsynonymous coding single nucleotide polymorphisms (cSNPs) and four synonymous cSNPs with a minor allele frequency greater than 5%. Functional studies showed that SNPs encoding Phe(65)Val and Asp(687)Glu displayed slightly increased levels of protein compared with WT, and Asp(687)Glu also caused increased GR receptor number. In addition, Ala(229)Thr and Ile(292)Val showed slightly decreased ligand binding affinity in COS-1 cells. A genotype-phenotype association study of NR3C1 gene expression in 240 lymphoblastoid cell lines identified one SNP, Cm746T>C, located 5'-upstream of noncoding exon 1C, and one haplotype, Cm237delC/Cm238C>T/Cm240G>C in exon 1C of the gene that were associated with GR mRNA expression and a trend with GR number.

Conclusions: These results represent a step toward understanding the functional role of common sequence variation in the GR gene (NR3C1) and the potential application of those SNPs in translational studies.