help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Journal of Clinical Endocrinology & Metabolism , doi:10.1210/jc.2007-0931
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints, Permissions and Rights
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marselli, L.
Right arrow Articles by Weir, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marselli, L.
Right arrow Articles by Weir, G. C.
Related Collections
Right arrow Diabetes and Insulin
The Journal of Clinical Endocrinology & Metabolism Vol. 93, No. 3 1046-1053
Copyright © 2008 by The Endocrine Society

Gene Expression of Purified β-Cell Tissue Obtained from Human Pancreas with Laser Capture Microdissection

Lorella Marselli, Jeffrey Thorne, Yu-Bae Ahn, Abdulkadir Omer, Dennis C. Sgroi, Towia Libermann, Hasan H. Otu, Arun Sharma, Susan Bonner-Weir and Gordon C. Weir

Section on Islet Transplantation and Cell Biology (L.M., J.T., Y.-B.A., A.O., A.S., S.B.-W., G.C.W.), Research Division, Joslin Diabetes Center and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215; Molecular Pathology Unit (D.C.S.), Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114; and Beth Israel Deaconess Medical Center (T.L., H.H.O.), Harvard Medical School, Boston, Massachusetts 02215

Address all correspondence and requests for reprints to: Gordon C. Weir, M.D., Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, One Joslin Place, Boston, Massachusetts 02215. E-mail: gordon.weir{at}joslin.harvard.edu.

Context: Human β-cell gene profiling is a powerful tool for understanding β-cell biology in normal and pathological conditions. Assessment is complicated when isolated islets are studied because of contamination by non-β-cells and the trauma of the isolation procedure.

Objective: The objective was to use laser capture microdissection (LCM) of human β-cells from pancreases of cadaver donors and compare their gene expression with that of handpicked isolated islets.

Design: Endogenous autofluorescence of β-cells facilitated procurement of purified β-cell tissue from frozen pancreatic sections with LCM. Gene expression profiles of three microdissected β-cell samples and three isolated islet preparations were obtained. The array data were normalized using DNA-Chip Analyzer software (Harvard School of Public Health, Boston, MA), and the lower confidence bound evaluated differentially expressed genes. Real-time PCR was performed on selected acinar genes and on the duct cell markers, carbonic anhydrase II and keratin 19.

Results: Endogenous autofluorescence facilitates the microdissection of β-cell rich tissue in human pancreas. When compared with array profiles of purified β-cell tissue, with lower confidence bound set at 1.2, there were 4560 genes up-regulated and 1226 genes down-regulated in the isolated islets. Among the genes up-regulated in isolated islets were pancreatic acinar and duct genes, chemokine genes, and genes associated with hypoxia, apoptosis, and stress. Quantitative RT-PCR confirmed the differential expression of acinar gene transcripts and the duct marker carbonic anhydrase II in isolated islets.

Conclusion: LCM makes it possible to obtain β-cell enriched tissue from human pancreas sections without the trauma and ischemia of islet isolation.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Borowiec, C. W. Liew, R. Thompson, W. Boonyasrisawat, J. Hu, W. M. Mlynarski, I. El Khattabi, S.-H. Kim, L. Marselli, S. S. Rich, et al.
Mutations at the BLK locus linked to maturity onset diabetes of the young and {beta}-cell dysfunction
PNAS, August 25, 2009; 106(34): 14460 - 14465.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
Y. Imai, H. R. Patel, N. M. Doliba, F. M. Matschinsky, J. W. Tobias, and R. S. Ahima
Analysis of gene expression in pancreatic islets from diet-induced obese mice
Physiol Genomics, December 12, 2008; 36(1): 43 - 51.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
Z. T. Bloomgarden
American College of Endocrinology Pre-Diabetes Consensus Conference: Part Three
Diabetes Care, December 1, 2008; 31(12): 2404 - 2409.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Boni-Schnetzler, J. Thorne, G. Parnaud, L. Marselli, J. A. Ehses, J. Kerr-Conte, F. Pattou, P. A. Halban, G. C. Weir, and M. Y. Donath
Increased Interleukin (IL)-1{beta} Messenger Ribonucleic Acid Expression in {beta}-Cells of Individuals with Type 2 Diabetes and Regulation of IL-1{beta} in Human Islets by Glucose and Autostimulation
J. Clin. Endocrinol. Metab., October 1, 2008; 93(10): 4065 - 4074.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2008 by The Endocrine Society