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Catecholamine Regulation of Local Lactate Production in Vivo in Skeletal Muscle and Adipose Tissue: Role of β-Adrenoreceptor Subtypes

Department of Medicine (V.Q., E.H.-T., J.B.), Karolinska University Hospital-Huddinge, Karolinska Institutet, SE-141 86 Stockholm, Sweden; and Department of Vascular Surgery (S.E.), Karolinska University Hospital-Solna, Karolinska Institutet, SE-171 76 Stockholm, Sweden

Address all correspondence and requests for reprints to: Veronica Qvisth, M.D., Ph.D., Diabetesmottagningen, Ersta Hospital, Box 4622, S-116 91 Stockholm, Sweden. E-mail: veronica.qvisth{at}ki.se.

Context: The regulation of lactate production in skeletal muscle (SM) and adipose tissue (AT) is not fully elucidated.

Objective: Our objective was to investigate the catecholamine-mediated regulation of lactate production and blood flow in SM and AT in healthy, normal-weight subjects by using microdialysis.

Methods: First, lactate levels in SM and AT were measured during an iv norepinephrine infusion (n = 11). Local blood flow was determined with the ¹³³Xe-clearance technique. Second, muscle lactate was measured during hypoglycemia and endogenous epinephrine stimulation (n = 12). Third, SM was perfused with selective β_1–3-adrenoreceptor agonists in situ (n = 8). Local blood flow was measured with the ethanol perfusion technique.

Results: In response to iv norepinephrine, the fractional release of lactate (difference between tissue and arterial lactate) increased by 40% in SM (P = 0.001), whereas remaining unchanged in AT. Blood flow decreased by 40% in SM (P < 0.005) and increased by 50% in AT (P < 0.05). In response to hypoglycemia, epinephrine increased 10-fold, and the fractional release of lactate in SM doubled (P < 0.0001). The blood flow remained unchanged. The β₂-agonist, terbutaline, caused a marked concentration-dependent increase of muscle lactate and blood flow (P < 0.0001). The β₁-agonist, dobutamine, induced a discrete increase of muscle lactate (P < 0.0001), and the blood flow remained unchanged. The β₃-agonist, CPG 12177, did not affect muscle lactate or blood flow.

Conclusions: Catecholamines stimulate lactate production in SM, but not in AT. In SM, the β₂-adrenoreceptor is the most important β-adrenergic receptor subtype in the regulation of lactate production.

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