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Nerve Growth Factor-Dependent Activation of trkA Receptors in the Human Ovary Results in Synthesis of Follicle-Stimulating Hormone Receptors and Estrogen Secretion

Laboratorio de Endocrinología y Biología Reproductiva, Departamento de Obstetricia y Ginecología, Hospital Clínico J. J. Aguirre, Universidad de Chile (C.S., M.J.-P., M.V., C.R.), Santiago 838-0456, Chile; Instituto de Investigaciones Materno Infantil, Escuela de Medicina, Universidad de Chile (R.P., M.V.), Santiago 838-0456, Chile; Oregon National Primate Research Center (C.M., B.K., S.R.O.), Beaverton, Oregon 97006; and Laboratorio de Neurobioquímica, Departamento Bioquímica y Biología Molecular, Facultad de Ciencias Quimicas y Farmacéuticas, Universidad de Chile (H.E.L.), Santiago 838-0456, Chile

Address correspondence and reprint requests to: Dr. Carmen Romero, Laboratorio de Endocrinología y Biología Reproductiva, Departamento Obstetricia y Ginecología, Facultad de Medicina, Hospital Clinico J. J. Aguirre, Universidad de Chile, Santos Dumont 999 Independencia, Santiago 838-0456, Chile. E-mail: cromero{at}redclinicauchile.cl.

Context: Previous studies showed that nerve growth factor (NGF) induces the expression of functional FSH receptors (FSHR) in preantral follicles of the developing rat ovary.

Objective: The objective of this study was to determine whether NGF can affect granulosa cell (GC) function in human periovulatory follicles using intact human ovaries and isolated human GCs.

Patients and Interventions: Human GCs were obtained from in vitro fertilization patients and normal ovaries from women with elective pelvic surgery for nonovarian indications.

Results: In normal ovaries, NGF and trkA (NGF’s high-affinity receptor) were detected by immunohistochemistry in GCs of preantral and antral follicles. NGF and trkA are also present in thecal cells of antral follicles. Both freshly collected and cultured GCs contained immunoreactive NGF and trkA in addition to their respective mRNAs. Human GCs respond to NGF with increased estradiol (E₂) secretion and a reduction in progesterone output. Exposure of human GCs to NGF increased FSHR mRNA content within 18 h of treatment, and this effect was blocked by the trk tyrosine kinase blocker K-252a. Also, cells preexposed to NGF released significantly more E₂ in response to hFSH than cells not pretreated with the neurotropin, showing that the NGF-induced increase in FSHR gene expression results in the formation of functional FSHRs.

Conclusions: These results suggest that one of the functions of NGF in the preovulatory human ovary is to increase the secretion of E₂ while preventing early luteinization via an inhibitory effect on progesterone secretion. NGF stimulates E₂ secretion both directly and by increasing the formation of FSHRs.

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