This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taleb, S. Articles by Clement, K. Search for Related Content PubMed PubMed Citation Articles by Taleb, S. Articles by Clement, K. Related Collections Metabolism Obesity

Weight Loss Reduces Adipose Tissue Cathepsin S and Its Circulating Levels in Morbidly Obese Women

Institut National de la Santé et de la Recherche Médicale U755, 75004 Paris, France; Université Pierre et Marie Curie-Paris 6, Faculté de Médecine, 75004 Paris, France; Centre Hospitalier Régional Universitaire, Pitié Salpétrière, Service de Nutrition, Hôtel Dieu (S.T., R.C., C.P., C.R., A.B., M.G.-M., D.L., K.C.), 75004 Paris, France; Surgery Department, Hôtel Dieu Hospital, Assistance Publique Hôpitaux de Paris (P.S., P.L., J.-L.B.), Paris, France; and Biochemistry Department, Hôtel Dieu Hospital, Assistance Publique Hôpitaux de Paris (C.C.), Paris, France

Address all correspondence and requests for reprints to: Dr. Karine Clément, Institut National de la Santé et de la Recherche Médicale U755, Nutrition Department, Hôtel Dieu, Place du parvis Notre Dame, 75004 Paris, France. E-mail: karine.clement{at}htd.ap-hop-paris.fr.

Context: Human adipose tissue produces several adipokines, including the newly identified protein cathepsin S (CTSS), a cysteine protease involved in the pathogenesis of atherosclerosis. Obesity is characterized by high levels of CTSS in the circulation and in sc white adipose tissue (scWAT).

Objective: We investigated the effect of surgery-induced weight loss on circulating CTSS and its protein expression in scWAT.

Design: Fifty morbidly obese women before and 3 months after surgery and 10 healthy lean women were studied. We analyzed the relationships between circulating CTSS and clinical and biological parameters. Immunohistochemistry of the CTSS protein variations in scWAT was performed.

Results: Weight loss decreased by 42% (P < 0.0001) the circulating CTSS levels, which correlated with changes in body weight (P = 0.03). We observed a significant decrease in CTSS enzymatic activity by 25% after weight loss (P = 0.001). Adipose tissue CTSS content was reduced by 30% (P = 0.002) after surgery. The variations in CTSS expression in scWAT after surgery correlated with changes in circulating CTSS serum levels (P = 0.03). Most of the correlations between CTSS and clinical and biological parameters disappeared after adjustment for body mass index, emphasizing the strong link between CTSS and corpulence in humans.

Conclusions: Changes in CTSS scWAT might contribute to serum variations in CTSS during weight loss. The decrease in CTSS concentrations in the circulation may contribute to vascular improvement in obese subjects after weight loss.

This article has been cited by other articles:

				M. Yang, J. Sun, T. Zhang, J. Liu, J. Zhang, M. A. Shi, F. Darakhshan, M. Guerre-Millo, K. Clement, B. D. Gelb, et al. Deficiency and Inhibition of Cathepsin K Reduce Body Weight Gain and Increase Glucose Metabolism in Mice Arterioscler. Thromb. Vasc. Biol., December 1, 2008; 28(12): 2202 - 2208. [Abstract] [Full Text] [PDF]

				S. Taleb, R. Cancello, K. Clement, and D. Lacasa Cathepsin S Promotes Human Preadipocyte Differentiation: Possible Involvement of Fibronectin Degradation Endocrinology, October 1, 2006; 147(10): 4950 - 4959. [Abstract] [Full Text] [PDF]