Endometrial Endothelial Cell Steroid Receptor Expression and Steroid Effects on Gene Expression
Graciela Krikun,
Frederick Schatz,
Robert Taylor,
Hilary O. D. Critchley,
Peter A. W. Rogers,
Joseph Huang and
Charles J. Lockwood
Department of Obstetrics, Gynecology, and Reproductive Sciences (G.K., F.S., J.H., C.J.L.), Yale University School of Medicine, New Haven, Connecticut 06520-8063; Center for Reproductive Sciences (R.T.), University of California, San Francisco, California 94143; Reproductive and Developmental Sciences (H.O.D.C.), Centre for Reproductive Biology, University of Edinburgh, Edinburgh EH16 4SB, United Kingdom; and Centre for Women’s Health Research (P.A.W.R.), Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, Clayton, Victoria 3168, Australia
Address all correspondence and requests for reprints to: Dr. Graciela Krikun, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, P.O. Box 208063, New Haven, Connecticut 06520-8063. E-mail: graciela.krikun{at}yale.edu.
Controversy exists regarding the expression of specific steroidreceptor proteins and mRNA in human microvascular endometrialendothelial cells (HEECs). Thus, we studied steroid receptorexpression in early passaged HEEC cultures and freshly isolatedHEECs. Analysis of estrogen receptor (ER) and progesterone receptor(PR) mRNA levels was carried out with real-time quantitativeRT-PCR, and the repertoire of genes activated by their respectivesteroid ligands was assessed by mRNA microarray analyses of18,400 genes and expressed sequence tags. We observed that culturedand freshly isolated HEECs each express ER-ß mRNAbut not ER-. In addition, PR mRNA was also detectable in bothHEEC sources. Microarray analysis demonstrated that treatmentof HEEC cultures with either estradiol or medroxyprogesteroneacetate produced differential effects on a wide variety of genes,and cluster analysis demonstrated that many of the genes areinvolved in intracellular signaling and enzymatic pathways.Thus, quantitative RT-PCR and microarray analyses demonstratethat HEECs express ER-ß and PR mRNA and that geneexpression by HEECs is differentially regulated by treatmentwith estrogen or progestin.
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. In addition, PR mRNA was also detectable in both HEEC sources. Microarray analysis demonstrated that treatment of HEEC cultures with either estradiol or medroxyprogesterone acetate produced differential effects on a wide variety of genes, and cluster analysis demonstrated that many of the genes are involved in intracellular signaling and enzymatic pathways. Thus, quantitative RT-PCR and microarray analyses demonstrate that HEECs express ER-ß and PR mRNA and that gene expression by HEECs is differentially regulated by treatment with estrogen or progestin. 
