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Aurora B Overexpression Associates with the Thyroid Carcinoma Undifferentiated Phenotype and Is Required for Thyroid Carcinoma Cell Proliferation

Dipartimenti di Biologia e Patologia Cellulare e Molecolare (R.S., S.Lib., P.L.P., D.S.-C., A.F., G.P.), Scienze Biomorfologiche e Funzionali-Istituto di Anatomia Patologica (G.T., L.P.), and Chimica Biologica (P.L.), Università Federico II, 80131 Naples, Italy; Cancer Research U.K. Centre for Cancer Therapeutics (V.B., S.Lin.), Institute of Cancer Research 15, Belmont, Sutton, Surrey SM2 5NG, United Kingdom; The Breakthrough Breast Cancer Research Centre (S.Lin.), Institute of Cancer Research, London SW3 6JB, United Kingdom; and Dipartimento di Medicina Sperimentale (P.C.), II Università di Napoli, 80138 Naples, Italy

Address all correspondence and requests for reprints to: Giuseppe Portella, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina e Chirurgia di Napoli, Università Federico II, via S. Pansini 5, 80131 Napoli, Italy. E-mail: portella{at}unina.it

Alterations in chromosome number (aneuploidy) are common in human neoplasias. Loss of mitotic regulation is believed to induce aneuploidy in cancer cells and act as a driving force during the malignant progression. The serine/theronine protein kinases of aurora family genes play a critical role in the regulation of key cell cycle processes. Aurora B mediates chromosome segregation by ensuring orientation of sister chromatids and overexpression of Aurora B in diploid human cells NHDF (normal human diploid fibroblast) induces multinuclearity.

We analyzed Aurora B expression in human thyroid carcinomas. Cell lines originating from different histotypes showed an increase in Aurora B expression. Immunohistochemical analysis of archive samples showed a high expression of Aurora B in anaplastic thyroid carcinomas; conversely, Aurora B expression was not detectable in normal thyroid tissue. Real-time PCR analysis confirmed a strong expression of Aurora B in anaplastic thyroid carcinomas.

The block of Aurora B expression induced by RNA interference or by using an inhibitor of Aurora kinase activity significantly reduced the growth of thyroid anaplastic carcinoma cells.

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