This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duffy, D. M. Articles by Seachord, C. L. Search for Related Content PubMed PubMed Citation Articles by Duffy, D. M. Articles by Seachord, C. L. Related Collections Female Endocrinology

Prostaglandin Dehydrogenase and Prostaglandin Levels in Periovulatory Follicles: Implications for Control of Primate Ovulation by Prostaglandin E2

Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia 23507

Address all correspondence and requests for reprints to: Diane M. Duffy, Department of Physiological Sciences, Eastern Virginia Medical School, 700 Olney Road, Lewis Hall, Norfolk, Virginia 23507. E-mail: duffydm{at}evms.edu.

Prostaglandin (PG) E2 produced by the periovulatory follicle in response to the midcycle LH surge is essential for successful ovulation in primates. Granulosa cells express the PG synthesis enzyme cyclooxygenase-2 in response to the LH surge, but elevated cyclooxygenase-2 mRNA levels precede rising follicular fluid PGE2 levels by 24 h. Therefore, PG metabolism may play a significant role in regulating follicular concentrations of PGE2 during the periovulatory interval. To test this hypothesis, granulosa cells, follicular fluid, and whole ovaries were obtained from adult monkeys receiving exogenous gonadotropins to stimulate development of multiple, large follicles at times spanning the 40-h periovulatory interval. Ovarian expression of the NAD+-dependent 15-hydroxy PG dehydrogenase (PGDH) was assessed by RT-PCR, Western blotting, and immunohistochemistry. PGDH mRNA levels were low in granulosa cells obtained 0 h after hCG, rose 10-fold 12 h after hCG, and were not different from 0 h by 24–36 h after hCG administration. Granulosa cell PGDH protein was present 0–12 h after hCG but was low/nondetectable 36 h after hCG administration. Follicular fluid PGE2 levels were low at 0–12 h, slightly higher at 24 h, and then rose 10-fold to peak at 36 h hCG. Levels of biologically inactive PGE2 metabolites in follicular fluid were also low at 0 h but elevated at 12–24 h after hCG, times at which PGE2 levels remain low. Therefore, PGDH is present in the primate periovulatory follicle in a pattern consistent with modulation of follicular PGE2 levels during the periovulatory interval, supporting the hypothesis that gonadotropin-regulated PGDH plays a role in the control and timing of ovulation in primates.

This article has been cited by other articles:

				M. THILL, S. BECKER, D. FISCHER, T. CORDES, A. HORNEMANN, K. DIEDRICH, D. SALEHIN, and M. FRIEDRICH Expression of Prostaglandin Metabolising Enzymes COX-2 and 15-PGDH and VDR in Human Granulosa Cells Anticancer Res, September 1, 2009; 29(9): 3611 - 3618. [Abstract] [Full Text] [PDF]

				N. Markosyan and D. M. Duffy Prostaglandin E2 Acts via Multiple Receptors to Regulate Plasminogen-Dependent Proteolysis in the Primate Periovulatory Follicle Endocrinology, January 1, 2009; 150(1): 435 - 444. [Abstract] [Full Text] [PDF]

				B. L Dozier, K. Watanabe, and D. M Duffy Two pathways for prostaglandin F2{alpha} synthesis by the primate periovulatory follicle Reproduction, July 1, 2008; 136(1): 53 - 63. [Abstract] [Full Text] [PDF]

				M. Sasseville, N. Cote, C. Vigneault, C. Guillemette, and F. J. Richard 3'5'-Cyclic Adenosine Monophosphate-Dependent Up-Regulation of Phosphodiesterase Type 3A in Porcine Cumulus Cells Endocrinology, April 1, 2007; 148(4): 1858 - 1867. [Abstract] [Full Text] [PDF]

				Q. Li, F. Jimenez-Krassel, A. Bettegowda, J. J Ireland, and G. W Smith Evidence that the preovulatory rise in intrafollicular progesterone may not be required for ovulation in cattle J. Endocrinol., March 1, 2007; 192(3): 473 - 483. [Abstract] [Full Text] [PDF]

				K. Sayasith, N. Bouchard, M. Dore, and J. Sirois Cloning of equine prostaglandin dehydrogenase and its gonadotropin-dependent regulation in theca and mural granulosa cells of equine preovulatory follicles during the ovulatory process Reproduction, February 1, 2007; 133(2): 455 - 466. [Abstract] [Full Text] [PDF]

				N. Markosyan, B. L. Dozier, F. A. Lattanzio, and D. M. Duffy Primate Granulosa Cell Response via Prostaglandin E2 Receptors Increases Late in the Periovulatory Interval Biol Reprod, December 1, 2006; 75(6): 868 - 876. [Abstract] [Full Text] [PDF]

				S. Ledoux, D. B. Campos, F. L. Lopes, M. Dobias-Goff, M.-F. Palin, and B. D. Murphy Adiponectin Induces Periovulatory Changes in Ovarian Follicular Cells Endocrinology, November 1, 2006; 147(11): 5178 - 5186. [Abstract] [Full Text] [PDF]

				D. M. Duffy, C. L. Seachord, and B. L. Dozier An Ovulatory Gonadotropin Stimulus Increases Cytosolic Phospholipase A2 Expression and Activity in Granulosa Cells of Primate Periovulatory Follicles J. Clin. Endocrinol. Metab., October 1, 2005; 90(10): 5858 - 5865. [Abstract] [Full Text] [PDF]

				D. M. Duffy, C. L. Seachord, and B. L. Dozier Microsomal prostaglandin E synthase-1 (mPGES-1) is the primary form of PGES expressed by the primate periovulatory follicle Hum. Reprod., June 1, 2005; 20(6): 1485 - 1492. [Abstract] [Full Text] [PDF]

				C. L. Seachord, C. A. VandeVoort, and D. M. Duffy Adipose Differentiation-Related Protein: A Gonadotropin- and Prostaglandin-Regulated Protein in Primate Periovulatory Follicles Biol Reprod, June 1, 2005; 72(6): 1305 - 1314. [Abstract] [Full Text] [PDF]