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Effect of Endurance Training on Adrenergic Control of Lipolysis in Adipose Tissue of Obese Women

Franco-Czech Laboratory for Clinical Research on Obesity, French Institute of Health and Medical Research (Institut National de la Santé et de la Recherche Médicale, Unité 586), and Third Faculty of Medicine, Charles University (B.R., V.S., C.M., J.P., E.K., M.M., I.H., N.V., F.C., D.L., M.L., M.B.), Prague, Czech Republic; Unité de Recherches sur les Obésités (Institut National de la Santé et de la Recherche Médicale, Unité 586), Institut Louis Bugnard, Centre Hospitalier Universitaire de Toulouse (C.M., I.H., N.V., F.C., D.L., M.L., M.B.), Université Paul Sabatier, 31403 Toulouse, France; Department of Sport Medicine and Obesity Unit, Third Faculty of Medicine, Charles University (B.R., V.S., J.P., E.K., M.M.), 10000 Prague, Czech Republic; and Laboratory of Medical and Clinical Pharmacology, Faculty of Medicine, Purpan Hospital (M.B.), 31073 Toulouse, France

Address all correspondence and requests for reprints to: Dr. Michel Berlan, Institut National de la Santé et de la Recherche Médicale, Unité 586, Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, 37 allées Jules Guesde, 31073 Toulouse Cedex, France. E-mail: berlan{at}cict.fr.

The effect of a 12-wk training program on sc abdominal adipose tissue (SCAAT) was studied in 11 obese women. Before and after the training, biopsies of SCAAT were performed for mRNA levels determination. Using the microdialysis method, involvement of ₂- and ß-adrenergic receptor (ARs) in the control of lipolysis in SCAAT was studied using local perfusion of epinephrine alone or supplemented with phentolamine, an ₂-AR antagonist. In addition, the variation in dialysate glycerol concentrations during exercise (50% peak oxygen consumption at 40 min) in a probe perfused with Ringer’s solution was compared with that obtained in a probe perfused with Ringer’s solution plus phentolamine. Training did not promote changes in the expression of key genes of the lipolytic pathway. The epinephrine-induced rise in the dialysate glycerol concentration was identical before and after training and was similarly potentiated by phentolamine. During exercise, the potentiating effect of phentolamine on the glycerol response was apparent before, but not after, training. The exercise-induced increase in plasma norepinephrine was lower after training (P = 0.04). In conclusion, training did not modify either the expression of genes involved in the control of lipolysis or ₂- and ß-ARs in situ sensitivity to epinephrine in SCAAT. Training reduced the antilipolytic action of catecholamines mediated by ₂-ARs during exercise, probably due to a reduction of exercise-induced catecholamine increase.

This work was supported by the Internal Grant Agency of Ministry of Health of Czech Republic (IGA 6836-3), Service of European Affairs (PECO Specific Action), French Ministry of F.A. (joint laboratory), and ALFEDIAM.

Abbreviations: AR, Adrenergic receptor; ATBF, adipose tissue blood flow; BMI, body mass index; NEFA, nonesterified fatty acid; SCAAT, sc abdominal adipose tissue; VO₂max, peak oxygen consumption.

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