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Hospices Civils de Lyon (P.C., G.R., N.Y., A.E.-B., M.P.), Fédération d’Endocrinologie du Pôle Est, Hôpital Neurologique Pierre Wertheimer, 69394 Lyon, France; and Laboratoire de Biologie Endocrinienne et Moléculaire (L.C.-M., Y.M.), Institut National de la Santé et de la Recherche Médicale U 418 (H.L.), and Equipes de Recherche et d’Innovations Technologiques ou Méthodologiques 0322 (P.C., G.R., A.E.-B., M.P.), Hôpital Debrousse, 29 rue Soeur Bouvier, 69322, Lyon, France
Address all correspondence and requests for reprints to: Michel Pugeat, Fédération d’Endocrinologie du Pole Est, Hôpital Neurologique Pierre Wertheimer, Aile A1 Bât HPGO, 59 Boulevard Pinel, 69394 Lyon Cedex 03, France. E-mail: michel.pugeat{at}chu-lyon.fr.
SHBG is the specific plasma transport protein for sex steroid hormones in humans. Plasma SHBG concentration follows a gender dimorphism but varies with nutritional and hormonal status in both sexes. In addition, a genetic influence on SHBG in humans has recently been suggested by family studies. We investigated the relationship between a point mutation (D327N) in SHBG gene exon 8 that delays human SHBG half-life and a pentanucleotide repeat polymorphism [PNRP (TAAAA)n] in the SHBG gene 5' untranslated region that influences transcription in vitro, on the one hand, and SHBG levels on the other, in a population of 303 women referred for hirsutism. Of these patients, 154 (51%) met the criteria for polycystic ovary syndrome (PCOS) and 124 (41%) were overweight [body mass index (BMI) 
25 kg/m2]. The two SHBG gene alleles for D327N substitution, wild-type (W) and variant (v), were identified by restriction fragment length polymorphism in the whole population, and the GeneScan method was used to identify PNRP alleles in 245 subjects. Six alleles of the pentanucleotide motif with six to 11 repeats were present in our population. Plasma SHBG concentration was related to PCOS status, non-SHBG-bound testosterone, BMI, fasting blood glucose level, fasting insulinemia, and D327N allele v. The v allele was associated with higher SHBG levels [36.9 ± 15.9 nmol/liter for W/v (n = 52) and 43.5 ± 3.5 nmol/liter for v/v (n = 2)] than was the wild-type W allele [31.1 ± 16.1 nmol/liter (n = 249); P = 0.039]. Multivariate analysis showed that BMI, PCOS status, and D327N polymorphism influenced plasma SHBG concentrations, each of these parameters contributing independently of the others.
Investigating the role of each allele of the TAAAA repeat polymorphism on SHBG levels was more complex because of the number of different genotypes (as many as 18 in our population) and the low frequency of some of them. Moreover, a strong disequilibrium linkage was found between D327N allele v and the eight-TAAAA repeat allele (P < 0.0001). This could mask the effect of the TAAAA repeat polymorphism on SHBG concentration in vivo. Nevertheless, SHBG levels in patients who were homozygous for six repeats (34.9 ± 16.2 nmol/liter; n = 21) were significantly (P = 0.043) higher than in nine-repeat homozygous patients (21.5 ± 13.0 nmol/liter; n = 8), and lay between the two for eight-repeat homozygous patients (28.5 ± 15.8 nmol/liter; n = 44). Delineating the precise role of this PNRP polymorphism will need further investigation in a large healthy population.
In summary, although BMI and PCOS status have a major influence on circulating SHBG levels in hirsute women, the present results support the notion that polymorphism(s) within the coding sequence and, potentially, in the regulatory sequence of the SHBG gene are associated with circulating SHBG levels and may represent part of the genetic background of sex steroid hormone activity in humans.
This work was supported by a generous grant from the Fondation de France; P.C. was supported by a postdoctoral fellowship from the Ligue Nationale Contre le Cancer.
This work was presented in part at the 84th Annual Meeting of The Endocrine Society, San Francisco, CA, 2002 (poster P607).
Abbreviations: A, Androstenedione; APO, apolipoprotein; BMI, body mass index; DHAS, dehydroepiandrosterone sulfate; Glc, glucose; GSTP, glutathione S-transferase; Ins, insulin; Lp, lipoprotein; PCOS, polycystic ovary syndrome; PNRP, pentanucleotide repeat polymorphism; T, testosterone; v, variant; W, wild-type.
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