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Effects of 3,5,3'-Triiodothyronine on the Invasive Potential and the Expression of Integrins and Matrix Metalloproteinases in Cultured Early Placental Extravillous Trophoblasts

Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan

Address all correspondence and requests for reprints to: Dr. Takeshi Maruo, Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. E-mail: maruo{at}kobe-u.ac.jp.

It is well known that T₃ plays a crucial role in the maintenance of early pregnancy through the induction of endocrine function in villous trophoblasts. The effects of T₃ on extravillous trophoblast (EVT) function, however, remain to be elucidated. To investigate the possible role of T₃ in the regulation of EVT invasion to the decidua, we have examined whether T₃ affects EVT invasive potential and the expression of matrix metalloproteinase-2 (MMP-2), MMP-3, tissue inhibitor metalloproteinase-1, fetal fibronectin (FN), and integrin ₅ß₁ in cultured early placental EVTs. Isolation and purification of trophoblasts differentiating into EVTs were performed by the enzymatic digestion of the anchoring chorionic villi, with the use of human FN-precoated culture dishes and FN-precoated Matrigel Transwells. The cells attached to the dishes were subcultured in DMEM supplemented with 10% fetal bovine serum for 48 h and were characterized by RT-PCR analysis after 24-h subculture and immunocytochemical analysis after 48-h subculture for specific EVT markers. Thereafter, the cultured cells were stepped down to a 4% fetal bovine serum condition and cultured in the presence or absence of T₃ (10^–8 M) for the subsequent 72 h. Matrigel invasion assay demonstrated that the treatment with T₃ significantly increased the number of cell projections of subsequent 24-, 48-, and 72-h cultured EVTs. RT-PCR analysis revealed that the treatment with T₃ increased the expression of MMP-2, MMP-3, fetal FN, and integrin ₅ß₁ mRNA in subsequent 24-h cultured EVTs compared with those in control cultures. Immunocytochemical and Western immunoblot analyses revealed that treatment with T₃ increased the expression of MMP-2 and MMP-3 in subsequent 48-h cultured EVTs compared with those in control cultures. The present results suggest that T₃ (10^–8 M) may play a vital role in up-regulating the invasive potential of EVTs into the decidua.

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