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Oxidative Stress Is Associated with Adiposity and Insulin Resistance in Men

Third Department of Internal Medicine (H.U., A.K., Y.S., E.C.G., K.M., N.M., N.K., T.T., Y.H., Y.Y., Y.A.), Department of Radiology (S.M.), and Department of Laboratory Medicine (K.N.), Mie University School of Medicine, Mie, 514-8507 Japan

Address all correspondence and requests for reprints to: Yasuhiro Sumida, M.D., Third Department of Internal Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. E-mail: sumidaya{at}clin.medic.mie-u.ac.jp.

To investigate the direct relationship of oxidative stress with obesity and insulin resistance in men, we measured the plasma levels of 8-epi-prostaglandin F2 (PGF2) in 14 obese and 17 nonobese men and evaluated their relationship with body mass index; body fat weight; visceral, sc, and total fat areas, measured by computed tomography; and glucose infusion rate during a euglycemic hyperinsulinemic clamp study.

Obese men had significantly higher plasma concentrations of 8-epi-PGF2 than nonobese men (P < 0.05). The plasma levels of 8-epi-PGF2 were significantly correlated with body mass index (r = 0.408; P < 0.05), body fat weight (r = 0.467; P < 0.05), visceral (r = 0.387; P < 0.05) and total fat area (r = 0.359; P < 0.05) in all (obese and nonobese) men. There was also a significant correlation between the plasma levels of 8-epi-PGF2 and glucose infusion rate in obese men (r = -0.552; P < 0.05) and all men (r = -0.668; P < 0.01). In all subjects, the plasma levels of 8-epi-PGF2 were significantly correlated with fasting serum levels of insulin (r = 0.487; P < 0.01).

In brief, these findings showed that the circulating levels of 8-epi-PGF2 are related to adiposity and insulin resistance in men. Although correlation does not prove causation, the results of this study suggest that obesity is an important factor for enhanced oxidative stress and that this oxidative stress triggers the development of insulin resistance in men.

H.U. and A.K. contributed equally to the completion of this investigation.

Abbreviations: BMI, Body mass index; FFA, free fatty acid(s); GIR, glucose infusion rate; OGTT, oral glucose tolerance test; PGF2, prostaglandin F2; ROS, reactive oxygen species.

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