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Urocortin in Human Gastric Mucosa: Relationship to Inflammatory Activity

Departments of Pharmacology (E.C., I.C., A.G.), Gastroenterology (C.L., I.A.M.), Pathology (M.T.), and Clinical Chemistry (C.T., A.N.M.), University of Crete School of Medicine, Heraklion 71110, Greece

Address all correspondence and requests for reprints to: Dr. A. Gravanis, Department of Pharmacology, University of Crete School of Medicine, Heraklion GR-71110, Crete, Greece. E-mail: gravanis{at}med.uoc.gr.

The presence of CRH and urocortin (Ucn), members of the CRH family of neuropeptides, was examined in human gastric biopsies from normal controls and in patients with active gastritis from Helicobacter pylori (H. pylori) and after eradication treatment. RT-PCR analysis showed the presence of the Ucn transcript in biopsies (obtained by gastroscopy) from normal and inflamed gastric mucosa, whereas the CRH transcript was not detectable. Immunoreactive (ir-) Ucn was localized (by immunohistochemistry) in gastric epithelial cells and in inflammatory elements of the surrounding negative for Ucn gastric stroma. The level of ir-Ucn was higher in gastric biopsies from the group of patients with active H. pylori gastritis than in normal controls (10.4 ± 1.8 vs. 2.0 ± 1.3 pg/µg total protein; P < 0.001). After the apparent eradication of H. pylori infection (by clinical and morphological criteria) ir-Ucn levels increased dramatically to 43.1 ± 9.8 pg/µg total protein, (P < 0.001) compared with pretreatment values. Interestingly, nonresponders to the eradication treatment did not show any significant change in ir-Ucn levels (18.7 ± 12.3 pg/µg total protein) compared with their pretreatment values. In conclusion, our data suggest that in human gastric epithelium Ucn is present and plays an important physiological role, whereas CRH is absent. In addition, and in contrast to what has been found for CRH in ulcerative colitis, a highly significant, but negative, correlation has been found between Ucn levels and gastric inflammation, suggesting that Ucn may exert an antiinflammatory effect in gastric mucosa.

A.N.M. and A.G. share senior authorship.

Abbreviations: CRH-R, CRH receptor; GI, gastrointestinal; ir-, immunoreactive; Ucn, urocortin.

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