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Luteinizing Hormone Pulsatility Is Disrupted at a Threshold of Energy Availability in Regularly Menstruating Women

Department of Biological Sciences, Ohio University, Athens, Ohio 45701

Address all correspondence and requests for reprints to: Anne B. Loucks, Department of Biological Sciences, Ohio University, Athens, Ohio 45701. E-mail: loucks{at}ohiou.edu.

To investigate the dependence of LH pulsatility on energy availability (dietary energy intake minus exercise energy expenditure), we measured LH pulsatility after manipulating the energy availability of 29 regularly menstruating, habitually sedentary, young women of normal body composition for 5 d in the early follicular phase. Subjects expended 15 kcal/kg of lean body mass (LBM) per day in supervised exercise at 70% of aerobic capacity while consuming a clinical dietary product to set energy availability at 45 and either 10, 20, or 30 kcal/kg LBM·d in two randomized trials separated by at least 2 months. Blood was sampled daily during treatments and at 10-min intervals for the next 24 h. Samples were assayed for LH, FSH, estradiol (E₂), glucose, ß-hydroxybutyrate, insulin, cortisol, GH, IGF-I, IGF-I binding protein (IGFBP)-1, IGFBP-3, leptin, and T₃. LH pulsatility was unaffected by an energy availability of 30 kcal/kg LBM·d (P > 0.3), but below this threshold LH pulse frequency decreased, whereas LH pulse amplitude increased (all P < 0.04). This disruption was more extreme in women with short luteal phases (P < 0.01). These incremental effects most closely resembled the effects of energy availability on plasma glucose, ß-hydroxybutyrate, GH, and cortisol and contrasted with the dependencies displayed by the other metabolic hormones (simultaneously P < 0.05). These results demonstrate that LH pulsatility is disrupted only below a threshold of energy availability deep into negative energy balance and suggest priorities for future investigations into the mechanism that mediates the nonlinear dependence of LH pulsatility on energy availability.

This research was supported in part by Grant DAMD 17-95-1-5053 from the U.S. Army Medical Research and Material Command (Defense Women’s Health & Military Medical Readiness Research Program); the General Clinical Research Branch, Division of Research Resources, NIH Grant M01 RR00034; and by Ross Laboratories. The content of the information reported in this paper does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

Abbreviations: BBT, Basal body temperature; CEE, controlled exercise energy expenditure; E₂, estradiol; 24EB, 24-h energy balance; 24EE, 24-h energy expenditure; ß-HOB; ß-hydroxybutyrate; IRMA, immunoradiometric; LBM, lean body mass; PO₂, pressure of O₂; RER, respiratory exchange ratio; VO_2max, maximum oxygen uptake.

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