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Autocrine Regulation of Human Prostate Carcinoma Cell Proliferation by Somatostatin through the Modulation of the SH2 Domain Containing Protein Tyrosine Phosphatase (SHP)-1

Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá (P.D.Z., R.M.R., A.M.V., R.M.M.-O., J.C.P., P.L.-R., B.C.), and Servicio de Urología, Hospital Universitario Príncipe de Asturias (J.A.), Alcalá de Henares-Madrid E-28871, Spain; INSERM, U-531, IFR 31, CHU Rangueil L3 (L.B., C.S.), Toulouse 31403, France; and Servicio de Anatomía Patológica, Hospital de Basurto, Universidad del País Vasco (J.I.L.), Bilbao E-48013, Spain

Address all correspondence and requests for reprints to: Dr. Begoña Colás, Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, E-28871 Alcalá de Henares-Madrid, Spain. E-mail: begona.colas{at}uah.es

Abstract

The present study was intended to gain additional information on the growth regulation of prostate by somatostatin (SRIF) and the intracellular events involved. The human prostate adenocarcinoma cell lines PC-3 and LNCaP produce SRIF and express subtypes 2 and 5 of SRIF receptors. The secretion of SRIF is related to the proliferative status of these cells; an inverse relationship exists between cell proliferation and the amount of secreted SRIF. Moreover, the growth of PC-3 cells is inhibited by SRIF overexpression and increased by blockage of endogenous SRIF. Coincident with the increase in SRIF secretion, the activity and levels of the SH2 domain containing protein tyrosine phosphatase (SHP)-1, present in PC-3 cells are augmented, but the effect can be partially prevented by neutralization of secreted endogenously SRIF. The activity of SHP-1 is also stimulated by the SRIF analog RC160. Overexpression of SHP-1 induces inhibition of PC-3 cell growth. SHP-1 is also present in normal prostate, benign prostatic hyperplasia, prostatic intraepithelial neoplasia, and well differentiated adenocarcinoma. In contrast, no signal is detected in poorly differentiated prostate cancer. These findings demonstrate that SRIF inhibits PC-3 and LNCaP cell proliferation through an autocrine/paracrine SRIF loop. This effect could be mediated by activation of the tyrosine phosphatase SHP-1 detected in these cells as well as in human prostate and prostate cancer.

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