This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Purchase Article View Shopping Cart Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Motoshima, H. Articles by Goldstein, B. J. Search for Related Content PubMed PubMed Citation Articles by Motoshima, H. Articles by Goldstein, B. J.

Differential Regulation of Adiponectin Secretion from Cultured Human Omental and Subcutaneous Adipocytes: Effects of Insulin and Rosiglitazone

Dorrance H. Hamilton Research Laboratories, Division of Endocrinology, Diabetes, and Metabolic Diseases, Department of Medicine (H.M., X.W., V.E.H., B.J.G.), and Department of Surgery (E.L.R., D.J.B., F.E.R.), Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107; and Linco Research, Inc. (M.K.S.), St. Charles, Missouri 63304

Address all correspondence and requests for reprints to: Barry J. Goldstein, M.D., Ph.D., Director, Division of Endocrinology and Metabolic Diseases, Jefferson Medical College, Room 349 Alumni Hall, 1020 Locust Street, Philadelphia, Pennsylvania 19107-6799. E-mail: barry.goldstein{at}mail.tju.edu.

Abstract

Adiponectin is an adipocyte-derived plasma protein with insulin-sensitizing and antiatherosclerotic properties. Because adipose tissue depots differ in the strength of their association with the adverse metabolic consequences of obesity, we studied the secretion of adiponectin in vitro from paired samples of isolated human omental and sc adipocytes and its regulation by insulin and rosiglitazone. Cells were incubated for 12 or 24 h with and without treatment with 100 nM insulin, 8 µM rosiglitazone, or both combined; adiponectin secreted into the culture medium was measured by a RIA with a human adiponectin standard and normalized for cellular DNA content. Secretion of adiponectin by omental cells was generally higher than sc cells and showed a strong negative correlation with body mass index (r = -0.78;P = 0.013). In contrast, secretion from the sc cells was unrelated to body mass index. Compared with sc-derived adipocytes, adiponectin secretion from omental cells was increased by insulin or rosiglitazone alone and was up to 2.3-fold higher following combined treatment with insulin and rosiglitazone, whereas secretion from sc adipose cells was unaffected by these treatments. These data suggest that reduced secretion from the omental adipose depot may account for the decline in plasma adiponectin observed in obesity. Furthermore, enhanced adiponectin secretion from fat cells derived from the visceral compartment in response to rosiglitazone alone or in combination with insulin may play a role in some of the systemic insulin-sensitizing and antiinflammatory properties of the thiazolidinediones.

This article has been cited by other articles:

				G. R. Hajer, T. W. van Haeften, and F. L.J. Visseren Adipose tissue dysfunction in obesity, diabetes, and vascular diseases Eur. Heart J., September 5, 2008; (2008) ehn387v1. [Abstract] [Full Text] [PDF]

				N. Stefan, K. Kantartzis, J. Machann, F. Schick, C. Thamer, K. Rittig, B. Balletshofer, F. Machicao, A. Fritsche, and H.-U. Haring Identification and Characterization of Metabolically Benign Obesity in Humans Arch Intern Med, August 11, 2008; 168(15): 1609 - 1616. [Abstract] [Full Text] [PDF]

				T. Hayashi, E. J. Boyko, M. J. McNeely, D. L. Leonetti, S. E. Kahn, and W. Y. Fujimoto Visceral Adiposity, Not Abdominal Subcutaneous Fat Area, Is Associated With an Increase in Future Insulin Resistance in Japanese Americans Diabetes, May 1, 2008; 57(5): 1269 - 1275. [Abstract] [Full Text] [PDF]

				L. Hojbjerre, M. Rosenzweig, F. Dela, J. M Bruun, and B. Stallknecht Acute exercise increases adipose tissue interstitial adiponectin concentration in healthy overweight and lean subjects Eur. J. Endocrinol., November 1, 2007; 157(5): 613 - 623. [Abstract] [Full Text] [PDF]

				A. J. G. Hanley, D. Bowden, L. E. Wagenknecht, A. Balasubramanyam, C. Langfeld, M. F. Saad, J. I. Rotter, X. Guo, Y.-D. I. Chen, M. Bryer-Ash, et al. Associations of Adiponectin with Body Fat Distribution and Insulin Sensitivity in Nondiabetic Hispanics and African-Americans J. Clin. Endocrinol. Metab., July 1, 2007; 92(7): 2665 - 2671. [Abstract] [Full Text] [PDF]

				D Kapoor, S Clarke, R Stanworth, K S Channer, and T H Jones The effect of testosterone replacement therapy on adipocytokines and C-reactive protein in hypogonadal men with type 2 diabetes Eur. J. Endocrinol., May 1, 2007; 156(5): 595 - 602. [Abstract] [Full Text] [PDF]

				S. H. Han, M. J. Quon, J.-a Kim, and K. K. Koh Adiponectin and Cardiovascular Disease: Response to Therapeutic Interventions J. Am. Coll. Cardiol., February 6, 2007; 49(5): 531 - 538. [Abstract] [Full Text] [PDF]

				E. W Demerath, W. Shen, M. Lee, A. C Choh, S. A Czerwinski, R. M Siervogel, and B. Towne Approximation of total visceral adipose tissue with a single magnetic resonance image Am. J. Clinical Nutrition, February 1, 2007; 85(2): 362 - 368. [Abstract] [Full Text] [PDF]

				A. D Sniderman, R. Bhopal, D. Prabhakaran, N. Sarrafzadegan, and A. Tchernof Why might South Asians be so susceptible to central obesity and its atherogenic consequences? The adipose tissue overflow hypothesis Int. J. Epidemiol., February 1, 2007; 36(1): 220 - 225. [Abstract] [Full Text] [PDF]

				T. Hayashi, E. J. Boyko, M. J. McNeely, D. L. Leonetti, S. E. Kahn, and W. Y. Fujimoto Minimum Waist and Visceral Fat Values for Identifying Japanese Americans at Risk for the Metabolic Syndrome Diabetes Care, January 1, 2007; 30(1): 120 - 127. [Abstract] [Full Text] [PDF]

				S. A. Phillips, O. A. Hatoum, and D. D. Gutterman The mechanism of flow-induced dilation in human adipose arterioles involves hydrogen peroxide during CAD Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H93 - H100. [Abstract] [Full Text] [PDF]

				J. Girard The Inhibitory Effects of Insulin on Hepatic Glucose Production Are Both Direct and Indirect Diabetes, December 1, 2006; 55(Supplement_2): S65 - S69. [Abstract] [Full Text] [PDF]

				N. Bansal, V. Charlton-Menys, P. Pemberton, P. McElduff, J. Oldroyd, A. Vyas, A. Koudsi, P. E. Clayton, J. K. Cruickshank, and P. N. Durrington Adiponectin in Umbilical Cord Blood Is Inversely Related to Low-Density Lipoprotein Cholesterol But Not Ethnicity J. Clin. Endocrinol. Metab., June 1, 2006; 91(6): 2244 - 2249. [Abstract] [Full Text] [PDF]

				K. K. Ong, J. Frystyk, A. Flyvbjerg, C. J. Petry, the Avon Longitudinal Study of Parents and Childre, A. Ness, and D. B. Dunger Sex-Discordant Associations With Adiponectin Levels and Lipid Profiles in Children. Diabetes, May 1, 2006; 55(5): 1337 - 1341. [Abstract] [Full Text] [PDF]

				J. L. Kuk, T. S. Church, S. N. Blair, and R. Ross Does Measurement Site for Visceral and Abdominal Subcutaneous Adipose Tissue Alter Associations With the Metabolic Syndrome? Diabetes Care, March 1, 2006; 29(3): 679 - 684. [Abstract] [Full Text] [PDF]

				S. Taleb, R. Cancello, C. Poitou, C. Rouault, P. Sellam, P. Levy, J.-L. Bouillot, C. Coussieu, A. Basdevant, M. Guerre-Millo, et al. Weight Loss Reduces Adipose Tissue Cathepsin S and Its Circulating Levels in Morbidly Obese Women J. Clin. Endocrinol. Metab., March 1, 2006; 91(3): 1042 - 1047. [Abstract] [Full Text] [PDF]

				M. Snijder, R. van Dam, M Visser, and J. Seidell What aspects of body fat are particularly hazardous and how do we measure them? Int. J. Epidemiol., February 1, 2006; 35(1): 83 - 92. [Full Text] [PDF]

				N. Rasouli, A. Yao-Borengasser, L. M. Miles, S. C. Elbein, and P. A. Kern Increased plasma adiponectin in response to pioglitazone does not result from increased gene expression Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E42 - E46. [Abstract] [Full Text] [PDF]

				D. G. Johns, Z. Ao, M. Eybye, A. Olzinski, M. Costell, S. Gruver, S. A. Smith, S. A. Douglas, and C. H. Macphee Rosiglitazone Protects against Ischemia/Reperfusion-Induced Leukocyte Adhesion in the Zucker Diabetic Fatty Rat J. Pharmacol. Exp. Ther., December 1, 2005; 315(3): 1020 - 1027. [Abstract] [Full Text] [PDF]

				L. B. Tanko, Y. Z. Bagger, G. Qin, P. Alexandersen, P. J. Larsen, and C. Christiansen Enlarged Waist Combined With Elevated Triglycerides Is a Strong Predictor of Accelerated Atherogenesis and Related Cardiovascular Mortality in Postmenopausal Women Circulation, April 19, 2005; 111(15): 1883 - 1890. [Abstract] [Full Text] [PDF]

				M. Cote, P. Mauriege, J. Bergeron, N. Almeras, A. Tremblay, I. Lemieux, and J.-P. Despres Adiponectinemia in Visceral Obesity: Impact on Glucose Tolerance and Plasma Lipoprotein and Lipid Levels in Men J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1434 - 1439. [Abstract] [Full Text] [PDF]

				V. Sepilian and M. Nagamani Adiponectin Levels in Women With Polycystic Ovary Syndrome and Severe Insulin Resistance Reproductive Sciences, February 1, 2005; 12(2): 129 - 134. [Abstract] [PDF]

				M. Grohmann, M. Sabin, J. Holly, J. Shield, E. Crowne, and C. Stewart Characterization of differentiated subcutaneous and visceral adipose tissue from children: the influences of TNF-{alpha} and IGF-I J. Lipid Res., January 1, 2005; 46(1): 93 - 103. [Abstract] [Full Text] [PDF]

				A. M. Delaigle, J.-C. Jonas, I. B. Bauche, O. Cornu, and S. M. Brichard Induction of Adiponectin in Skeletal Muscle by Inflammatory Cytokines: in Vivo and in Vitro Studies Endocrinology, December 1, 2004; 145(12): 5589 - 5597. [Abstract] [Full Text] [PDF]

				L. B. Tanko, J. M. Bruun, P. Alexandersen, Y. Z. Bagger, B. Richelsen, C. Christiansen, and P. J. Larsen Novel Associations Between Bioavailable Estradiol and Adipokines in Elderly Women With Different Phenotypes of Obesity: Implications for Atherogenesis Circulation, October 12, 2004; 110(15): 2246 - 2252. [Abstract] [Full Text] [PDF]

				C. Winzer, O. Wagner, A. Festa, B. Schneider, M. Roden, D. Bancher-Todesca, G. Pacini, T. Funahashi, and A. Kautzky-Willer Plasma Adiponectin, Insulin Sensitivity, and Subclinical Inflammation in Women With Prior Gestational Diabetes Mellitus Diabetes Care, July 1, 2004; 27(7): 1721 - 1727. [Abstract] [Full Text] [PDF]

				T. Hayashi, E. J. Boyko, D. L. Leonetti, M. J. McNeely, L. Newell-Morris, S. E. Kahn, and W. Y. Fujimoto Visceral Adiposity Is an Independent Predictor of Incident Hypertension in Japanese Americans Ann Intern Med, June 15, 2004; 140(12): 992 - 1000. [Abstract] [Full Text] [PDF]

				J. Sutinen, K. Kannisto, E. Korsheninnikova, R. M. Fisher, E. Ehrenborg, T. Nyman, A. Virkamaki, T. Funahashi, Y. Matsuzawa, H. Vidal, et al. Effects of rosiglitazone on gene expression in subcutaneous adipose tissue in highly active antiretroviral therapy-associated lipodystrophy Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E941 - E949. [Abstract] [Full Text] [PDF]

				S. Urs, C. Smith, B. Campbell, A. M. Saxton, J. Taylor, B. Zhang, J. Snoddy, B. Jones Voy, and N. Moustaid-Moussa Gene Expression Profiling in Human Preadipocytes and Adipocytes by Microarray Analysis J. Nutr., April 1, 2004; 134(4): 762 - 770. [Abstract] [Full Text] [PDF]

				J. Hoffstedt, E. Arvidsson, E. Sjolin, K. Wahlen, and P. Arner Adipose Tissue Adiponectin Production and Adiponectin Serum Concentration in Human Obesity and Insulin Resistance J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1391 - 1396. [Abstract] [Full Text] [PDF]

				F. Abbasi, J. W. Chu, C. Lamendola, T. McLaughlin, J. Hayden, G. M. Reaven, and P. D. Reaven Discrimination Between Obesity and Insulin Resistance in the Relationship With Adiponectin Diabetes, March 1, 2004; 53(3): 585 - 590. [Abstract] [Full Text] [PDF]

				H. Bays, L. Mandarino, and R. A. DeFronzo Role of the Adipocyte, Free Fatty Acids, and Ectopic Fat in Pathogenesis of Type 2 Diabetes Mellitus: Peroxisomal Proliferator-Activated Receptor Agonists Provide a Rational Therapeutic Approach J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 463 - 478. [Full Text] [PDF]

				M. Garaulet, N. Viguerie, S. Porubsky, E. Klimcakova, K. Clement, D. Langin, and V. Stich Adiponectin Gene Expression and Plasma Values in Obese Women during Very-Low-Calorie Diet. Relationship with Cardiovascular Risk Factors and Insulin Resistance J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 756 - 760. [Abstract] [Full Text] [PDF]

				M. B. Snijder, J. M. Dekker, M. Visser, L. M. Bouter, C. D.A. Stehouwer, J. S. Yudkin, R. J. Heine, G. Nijpels, and J. C. Seidell Trunk Fat and Leg Fat Have Independent and Opposite Associations With Fasting and Postload Glucose Levels: The Hoorn Study Diabetes Care, February 1, 2004; 27(2): 372 - 377. [Abstract] [Full Text] [PDF]

				F. Bacha, R. Saad, N. Gungor, and S. A. Arslanian Adiponectin in Youth: Relationship to visceral adiposity, insulin sensitivity, and {beta}-cell function Diabetes Care, February 1, 2004; 27(2): 547 - 552. [Abstract] [Full Text] [PDF]

				Z. T. Bloomgarden The 1st World Congress on the Insulin Resistance Syndrome Diabetes Care, February 1, 2004; 27(2): 602 - 609. [Full Text] [PDF]

				P. J. Havel Update on Adipocyte Hormones: Regulation of Energy Balance and Carbohydrate/Lipid Metabolism Diabetes, February 1, 2004; 53(90001): S143 - 151. [Abstract] [Full Text]

				M. Bajaj, S. Suraamornkul, P. Piper, L. J. Hardies, L. Glass, E. Cersosimo, T. Pratipanawatr, Y. Miyazaki, and R. A. DeFronzo Decreased Plasma Adiponectin Concentrations Are Closely Related to Hepatic Fat Content and Hepatic Insulin Resistance in Pioglitazone-Treated Type 2 Diabetic Patients J. Clin. Endocrinol. Metab., January 1, 2004; 89(1): 200 - 206. [Abstract] [Full Text] [PDF]

				E. Sivan, S. Mazaki-Tovi, C. Pariente, Y. Efraty, E. Schiff, R. Hemi, and H. Kanety Adiponectin in Human Cord Blood: Relation to Fetal Birth Weight and Gender J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 5656 - 5660. [Abstract] [Full Text] [PDF]

				Y.-M. Liu, J.-M. Lacorte, N. Viguerie, C. Poitou, V. Pelloux, B. Guy-Grand, C. Coussieu, D. Langin, A. Basdevant, and K. Clement Adiponectin Gene Expression in Subcutaneous Adipose Tissue of Obese Women in Response to Short-Term Very Low Calorie Diet and Refeeding J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 5881 - 5886. [Abstract] [Full Text] [PDF]

				A. Gavrila, J. L. Chan, N. Yiannakouris, M. Kontogianni, L. C. Miller, C. Orlova, and C. S. Mantzoros Serum Adiponectin Levels Are Inversely Associated with Overall and Central Fat Distribution but Are Not Directly Regulated by Acute Fasting or Leptin Administration in Humans: Cross-Sectional and Interventional Studies J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4823 - 4831. [Abstract] [Full Text] [PDF]