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Estrogen Receptor and ß Expression in Theca and Granulosa Cells from Women with Polycystic Ovary Syndrome

Department of Obstetrics and Gynecology (S.R.W., H.-W.Y., D.A.M.), Cedars-Sinai Burns and Allen Research Institute, Cedars-Sinai Medical Center/David Geffen School of Medicine at UCLA, Los Angeles, California 90048; and Department of Obstetrics and Gynecology (A.J.J., M.B.), Second Clinic of Surgical Gynecology, University School of Medicine, 20-090 Lublin, Poland

Address all correspondence and requests for reprints to: Denis A. Magoffin, Ph.D., Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Davis 2066, Los Angeles, California 90048. E-mail: magoffin{at}cshs.org.

Abstract

A defining characteristic of dominant follicles is high estradiol concentrations. Abnormal expression of estrogen receptors (ERs) could contribute to poor follicular development and ovulatory failure in polycystic ovary syndrome (PCOS). The aim of this study was to determine whether there are differences in ER and ERß expression in granulosa cells (GC) and theca cells (TC) from women with PCOS, compared with regularly cycling women. GC and TC were obtained by microdissection from 12 polycystic and 23 normal ovaries. ER and ERß mRNA and protein expression were measured by semiquantitative RT-PCR and Western blot, respectively. In control ovaries, both GC and TC ER mRNAs were higher in small antral (SA) than in dominant follicles. ER mRNA was similar in PCOS and size-matched control follicles. In control follicles, ER protein concentrations were higher in GC than in TC. In GC, the ER concentrations were comparable among SA, dominant, and PCOS follicles. In TC, ER concentrations were lower in dominant follicles but were markedly increased in PCOS. In control ovaries, GC and TC expression of ERß mRNA was higher in SA, compared with dominant follicles. In PCOS, ERß mRNA was intermediate between SA and dominant follicles in both GC and TC. In GC, the ERß protein concentrations followed the same pattern as mRNA expression; but in TC ERß, protein in PCOS was equivalent to that in dominant follicles. The results of this study demonstrate that there are significant alterations in the expression of ER and ERß in PCOS that may be related to abnormal follicular development.

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