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Circulating Soluble Leptin Receptor and Free Leptin Index during Childhood, Puberty, and Adolescence

Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics (J.K., A.L., B.S., J.T.), Hospital for Children and Adolescents (A.L., A.B., B.S., G.M., W.K.), University of Leipzig, 04103 Leipzig, Germany; and Department of Child and Adolescent Psychiatry of the University of Marburg (J.H.), 35039 Marburg, Germany

Address all correspondence and requests for reprints to: Dr. J. Kratzsch, Department of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Liebigstrasse 27a, D-04103 Leipzig, Germany. E-mail: Kraj{at}medizin.uni-leipzig.de.

Abstract

Leptin is bound in human blood by a high affinity binding protein, which appears to be identical with the soluble leptin receptor (sOB-R). Using a ligand-mediated immunofunctional assay for the determination of serum sOB-R, we investigated its course during childhood, puberty, and adolescence in a large cohort of 581 healthy children and adolescents and a small group of 13 patients with anorexia nervosa. In the first years of life, sOB-R is detectable in remarkably high concentrations. Thereafter, a continuous decline of sOB-R levels was found. Consequently, correlation analyses demonstrated significant inverse relationships (P < 0.001) of sOB-R with age, IGF-I levels, pubertal stage, auxological and body composition parameters, as well as with leptin concentrations. Multiple regression analysis revealed that height, IGF-I, and age (only in girls) were independent predictors of sOB-R levels; these variables account for approximately 65% and 48% of the variation of sOB-R levels in boys and girls, respectively. The courses of age-dependent median values for the free leptin index (FLI, ratio between leptin and sOB-R levels) and for leptin levels were parallel in both genders. Correlation analyses demonstrated that in particular parameters of growth and sexual maturation are more closely related to the FLI than to leptin alone; this closer relationship is more pronounced among boys. Weight gains of patients with anorexia nervosa resulted in a significant increase in leptin and IGF-I levels (P < 0.01), whereas the median of sOB-R values decreased (P < 0.01). sOB-R and IGF-I levels were again significantly correlated (r = -0.55, P < 0.01). These findings suggest that high levels of sOB-R in emaciation may reflect an up-regulation of the sOB-R to suppress leptin action during energy deficiency. Furthermore, determinations of sOB-R and FLI are additional valuable tools to investigate the leptin axis during growth and sexual maturation.

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