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The Journal of Clinical Endocrinology & Metabolism Vol. 86, No. 9 4552
Copyright © 2001 by The Endocrine Society

Rapid Communications

A Low Dose of Ghrelin Stimulates Growth Hormone (GH) Release Synergistically with GH-Releasing Hormone in Humans

Yuji Hataya, Takashi Akamizu, Kazuhiko Takaya, Naotetsu Kanamoto, Hiroyuki Ariyasu, Misa Saijo, Kenji Moriyama, Akira Shimatsu, Masayasu Kojima, Kenji Kangawa and Kazuwa Nakao

Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine (Y.H., T.A., K. T., N.K., H.A., K.H., M.S., K.M., K.N.), Kyoto 606-8507; Department of Biochemistry, National Cardiovascular Center Research Institute (M.K., K.K.), Osaka 565-8565; and Clinical Research Institute, Center for Endocrine and Metabolic Diseases, Kyoto National Hospital (A.S.), Kyoto 612-8555, Japan

Abstract

The synergistic relationship between GH-releasing secretagogue (GHS) and GH-releasing hormone (GHRH) with respect to GH secretion is well known. In the present study, we report a similar relationship between GHRH and ghrelin, a recently identified endogenous ligand for the GHS receptor. In normal male adults, various doses of ghrelin were intravenously administered alone or together with 1.0 µg/kg GHRH. At small doses of 0.08 and 0.2 µg/kg ghrelin, combined administration of the two peptides significantly stimulated GH release in a synergistic manner; the mean GH response values of the two peptide combinations were more than the summed mean GH response values of each peptide alone (P < 0.05). In addition, at 1.0 µg/kg ghrelin, the tendency of the synergistic effect was observed, although the comparison was not statistically significant probably due to a submaximal dose ceiling effect. No synergistic effects with respect to ACTH or prolactin secretion were observed. In conclusion, the synergistic interaction between ghrelin and GHRH was clearly shown and might be useful for a provocation test to diagnose GH deficiency.




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