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Expression of 11ß-Hydroxysteroid Dehydrogenase Isoenzymes in the Human Pituitary: Induction of the Type 2 Enzyme in Corticotropinomas and Other Pituitary Tumors

Departments of Endocrinology (M.K., J.N., A.B.G.) and Histopathology (S.J.), St. Bartholomew’s Hospital, London, United Kingdom EC1A 7BE; Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital (I.B., P.M.S.), Birmingham, United Kingdom B15 2TH UK; and Department of Internal Medicine, Toho University (M.S.), Tokyo, Japan 143-8541

Address all correspondence and requests for reprints to: Dr. Ashley Grossman, Department of Endocrinology, St. Bartholomew’s Hospital, West Smithfield, London, United Kingdom EC1A 7BE. E-mail: a.b.grossman{at}mds.qmw.ac.uk

Abstract

One of the defining biochemical features of Cushing’s disease is a relative insensitivity to glucocorticoid (GC) feedback, but an analysis of the GC receptor has failed to detect any major abnormalities. However, two isoenzymes of 11ß-hydroxysteroid dehydrogenase (11ßHSD), either by converting cortisone (E) to cortisol (F) (type 1) or conversely by converting F to E (type 2), play an important prereceptor role in regulating corticosteroid hormone action at several sites. 11ßHSD1 and -2 expression within the anterior pituitary gland itself may modulate GC feedback at an autocrine level, and we have speculated that this may be deranged in Cushing’s disease. Detection of 11ßHSD type 1 and 2 immunoreactive protein was performed using fluorescence immunohistochemistry. Double immunofluorescent studies were undertaken on normal pituitary to define the cellular localization of 11ßHSD isoenzymes using antisera against GH, ACTH, LH, FSH, PRL, and S100, a nonhormonal marker of folliculo-stellate cells. In normal pituitary, positive staining for 11ßHSD1-immunoreactive protein was observed in GH- and PRL-secreting cells and in folliculo-stellate cells; gonadotrophs, thyrotrophs, and ACTH-positive cells were negative. 11ßHSD2 immunoreactivity was absent in all cell types. RT-PCR detected 11ßHSD1 messenger ribonucleic acid (mRNA) expression in the normal pituitary; 11ßHSD2 mRNA expression was also seen in most normal tissue.

By contrast, in ACTH-secreting adenomas 11ßHSD2 immunostaining was strongly positive in every case of corticotroph adenoma. 11ßHSD1 immunoreactivity was also observed occasionally, but to a much lesser extent. In other pituitary tumors, both functional and nonfunctional, 11ßHSD expression was variable in terms of isoenzyme mRNA and intensity of protein staining. The expression of 11ßHSD1 (which generates F from E) in somatotrophs and lactotrophs suggests an autocrine role for this isoenzyme in the glucocorticoid regulation of pituitary GH and PRL secretion. 11ßHSD2 expression is markedly induced in ACTH-secreting pituitary tumors and, by converting F to E, may explain the resetting of glucocorticoid feedback control in Cushing’s disease.

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