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Estrogen Receptors and ß Are Differentially Expressed in Developing Human Bone¹

Address all correspondence and requests for reprints to: Dr. Sharyn Bord, University of Cambridge School of Clinical Medicine, Addenbrooke’s Hospital, Box 157, Cambridge, United Kingdom CB2 2QQ. E-mail: sb201{at}cam.ac.uk

Estrogen plays an essential role in the development and maintenance of the skeleton; its effects are mediated via interactions with two estrogen receptor (ER) subtypes, and ß. The aim of this study was to establish the cellular distribution of ER and ERß in neonatal human rib bone. ER and ERß immunoreactivity was seen in proliferative and prehypertrophic chondrocytes in the growth plate, with lower levels of expression in the late hypertrophic zone. Different patterns of expression of the two ERs were seen in bone. In cortical bone, intense staining for ER was observed in osteoblasts and osteocytes adjacent to the periosteal-forming surface and in osteoclasts on the opposing resorbing surface. In cancellous bone, ERß was strongly expressed in both osteoblasts and osteocytes, whereas only low expression of ER was seen in these areas. Nuclear and cytoplasmic staining for ERß was apparent in osteoclasts. These observations demonstrate distinct patterns of expression for the two ER subtypes in developing human bone and indicate functions in both the growth plate and mineralized bone. In the latter, ER is predominantly expressed in cortical bone, whereas ERß shows higher levels of expression in cancellous bone.

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