This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, H.-Y. Articles by Polan, M. L. Search for Related Content PubMed PubMed Citation Articles by Huang, H.-Y. Articles by Polan, M. L. Pubmed/NCBI databases Substance via MeSH

Interleukin (IL)-1ß Regulation of IL-1ß and IL-1 Receptor Antagonist Expression in Cultured Human Endometrial Stromal Cells¹

Department of Gynecology and Obstetrics (H.-Y.H., Y.W., J.S.K., F.R., M.L.P.), Stanford University School of Medicine, Stanford, California 94305; and Department of Obstetrics and Gynecology (H.-Y.H., Y.-K.S.), Lin-Kou Medical Center, Chang Gung Memorial Hospital and University School of Medicine, Taipei, Taiwan

Address all correspondence and requests for reprints to: Hong-Yuan Huang, Department of Obstetrics and Gynecology, Lin-Kou Medical Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan. E-mail: hykh{at}ms18.hinet.net

The interleukin (IL)-1 system is a major regulator of local cellular interactions during embryonic implantation. Because IL-1ß and IL receptor antagonist (IL-1ra) are both expressed in human endometrium, we hypothesized that an appropriate ratio of IL-1ß to IL-1ra might favor the process of embryo implantation. Therefore, we investigated IL-1 regulation of the quantitative ratio of IL-1ß/IL-1ra messenger RNA (mRNA) expression in human endometrial stromal cells using quantitative competitive PCR, as well as intracellular protein expression after stromal cell solubilization. Confluent stromal cell cultures were stimulated with human IL-1ß (0–1000 IU/mL) for 24 h. After 24 h, total RNA was extracted, reverse transcribed, and coamplified by PCR with a defined amount of internal standard. The quantitative ratio was determined by the density of target to the internal standard. After culture with IL-1ß for 24 and 48 h, stromal cells were solubilized, and the intracellular protein levels of IL-1ß and IL-1ra were measured by enzyme-linked immunosorbent assay. The IL-1ß and IL-1ra mRNA were both up-regulated, and IL-1R tI mRNA was down-regulated, by IL-1ß in a dose-dependent manner. The quantitative ratio of IL-1ß to IL-1ra mRNA was constant with the presence of increasing concentrations of IL-1ß (1–1000 IU/mL). IL-1ß and IL-1ra protein was not detected in conditioned media of cultures before addition of IL-1ß. IL-1ß and IL-1ra protein levels increased with increasing amounts of IL-1ß after solubilization of stromal cells. The IL-1ß was detectable after 12 h of culture, in comparison with IL-1ra, which was detectable after 24 h of IL-1ß stimulation. These results suggest that IL-1 may play a crucial role in embryo-maternal interaction by regulating stromal cell expression of IL-1ß and IL-1ra, resulting in an appropriate ratio during the process of embryonic implantation.

This article has been cited by other articles:

				A.A Fouladi-Nashta, L Mohamet, J.K Heath, and S.J Kimber Interleukin 1 Signaling Is Regulated by Leukemia Inhibitory Factor (LIF) and Is Aberrant in Lif-/- Mouse Uterus Biol Reprod, July 1, 2008; 79(1): 142 - 153. [Abstract] [Full Text] [PDF]

				M. Kimatrai, O. Blanco, R. Munoz-Fernandez, I. Tirado, F. Martin, A. C. Abadia-Molina, and E. G. Olivares Contractile Activity of Human Decidual Stromal Cells. II. Effect of Interleukin-10 J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6126 - 6130. [Abstract] [Full Text] [PDF]

				E. Dimitriadis, C.A. White, R.L. Jones, and L.A. Salamonsen Cytokines, chemokines and growth factors in endometrium related to implantation Hum. Reprod. Update, November 1, 2005; 11(6): 613 - 630. [Abstract] [Full Text] [PDF]

				M. Nishida, K. Nasu, J. Fukuda, Y. Kawano, H. Narahara, and I. Miyakawa Down-Regulation of Interleukin-1 Receptor Type 1 Expression Causes the Dysregulated Expression of CXC Chemokines in Endometriotic Stromal Cells: A Possible Mechanism for the Altered Immunological Functions in Endometriosis J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5094 - 5100. [Abstract] [Full Text] [PDF]

				K. Yuan, T.-M. Hong, J. J. W. Chen, W. H. Tsai, and M. T. Lin Syndecan-1 up-regulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis Blood, August 15, 2004; 104(4): 1025 - 1033. [Abstract] [Full Text] [PDF]

				N. Yamanaka, T. Morisaki, H. Nakashima, A. Tasaki, M. Kubo, H. Kuga, C. Nakahara, K. Nakamura, H. Noshiro, T. Yao, et al. Interleukin 1{beta} Enhances Invasive Ability of Gastric Carcinoma through Nuclear Factor-{kappa}B Activation Clin. Cancer Res., March 1, 2004; 10(5): 1853 - 1859. [Abstract] [Full Text] [PDF]

				T. E. Curry Jr. and K. G. Osteen The Matrix Metalloproteinase System: Changes, Regulation, and Impact throughout the Ovarian and Uterine Reproductive Cycle Endocr. Rev., August 1, 2003; 24(4): 428 - 465. [Abstract] [Full Text] [PDF]

				P. Vigano, E. Somigliana, S. Mangioni, M. Vignali, M. Vignali, and A. M. Di Blasio Expression of Interleukin-10 and Its Receptor Is Up-Regulated in Early Pregnant Versus Cycling Human Endometrium J. Clin. Endocrinol. Metab., December 1, 2002; 87(12): 5730 - 5736. [Abstract] [Full Text] [PDF]