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Interleukin-1ß Regulates Urokinase Plasminogen Activator (u-PA), u-PA Receptor, Soluble u-PA Receptor, and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acid Expression in Cultured Human Endometrial Stromal Cells¹

Department of Gynecology and Obstetrics, Stanford University School of Medicine (H.W.C., Y.W., M.L.P.), Stanford, California 94305-5317; Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine (H.W.C., J.J.A., H.S.M.), 158-710 Seoul, Korea

Address all correspondence and requests for reprints to: Dr. Hye-Won Chung, Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, 911-1 Yang Chun Ku Mock 6 Dong 158-710 Seoul, Korea. E-mail: hyewon{at}mm.ewha.ac.kr

The interleukin-1 (IL-1) system plays an integral role in local intercellular interactions during implantation. In addition, the plasminogen activator system, especially urokinase plasminogen activator (u-PA), plasminogen activator inhibitor (PAI-1), and u-PA receptor (u-PAR), are crucial during embryo implantation. Decidualization and implantation are complex processes dependent upon several proteases, including u-PA, and IL-1 is known to affect PA activity in several cell types. We investigated the role of IL-1ß in regulating u-PA, PAI-1, u-PAR, and soluble u-PAR messenger ribonucleic acid (mRNA) expression in cultured human endometrial stromal cells using quantitative competitive PCR. For confirmation of the mRNA data, we measured PAI-1 and u-PAR protein by enzyme-linked immunosorbent assay. Confluent stromal cell cultures treated with progesterone and estradiol for 9 days were stimulated with IL-1ß, and IL-1ß plus IL-1ß antibody for an additional 24 h. Total RNA was extracted, reverse transcribed, and coamplified using quantitative and competitive PCR with internal standards. IL-1ß increased PAI-1, u-PAR, and soluble u-PAR expression in a dose-dependent manner, and this result was reversed by anti-IL-1ß antibody treatment. u-PA mRNA expression was not dependent on IL-1ß. These results suggest that IL-1 may be important in regulating PAI-1 and u-PAR during stromal cell decidualization before implantation.

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