This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chung, H.-W. Articles by Polan, M. L. Search for Related Content PubMed PubMed Citation Articles by Chung, H.-W. Articles by Polan, M. L.

Interleukin-1ß Regulates Urokinase Plasminogen Activator (u-PA), u-PA Receptor, Soluble u-PA Receptor, and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acid Expression in Cultured Human Endometrial Stromal Cells¹

Department of Gynecology and Obstetrics, Stanford University School of Medicine (H.W.C., Y.W., M.L.P.), Stanford, California 94305-5317; Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine (H.W.C., J.J.A., H.S.M.), 158-710 Seoul, Korea

Address all correspondence and requests for reprints to: Dr. Hye-Won Chung, Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, 911-1 Yang Chun Ku Mock 6 Dong 158-710 Seoul, Korea. E-mail: hyewon{at}mm.ewha.ac.kr

The interleukin-1 (IL-1) system plays an integral role in local intercellular interactions during implantation. In addition, the plasminogen activator system, especially urokinase plasminogen activator (u-PA), plasminogen activator inhibitor (PAI-1), and u-PA receptor (u-PAR), are crucial during embryo implantation. Decidualization and implantation are complex processes dependent upon several proteases, including u-PA, and IL-1 is known to affect PA activity in several cell types. We investigated the role of IL-1ß in regulating u-PA, PAI-1, u-PAR, and soluble u-PAR messenger ribonucleic acid (mRNA) expression in cultured human endometrial stromal cells using quantitative competitive PCR. For confirmation of the mRNA data, we measured PAI-1 and u-PAR protein by enzyme-linked immunosorbent assay. Confluent stromal cell cultures treated with progesterone and estradiol for 9 days were stimulated with IL-1ß, and IL-1ß plus IL-1ß antibody for an additional 24 h. Total RNA was extracted, reverse transcribed, and coamplified using quantitative and competitive PCR with internal standards. IL-1ß increased PAI-1, u-PAR, and soluble u-PAR expression in a dose-dependent manner, and this result was reversed by anti-IL-1ß antibody treatment. u-PA mRNA expression was not dependent on IL-1ß. These results suggest that IL-1 may be important in regulating PAI-1 and u-PAR during stromal cell decidualization before implantation.

This article has been cited by other articles:

				H. Zhu, P.C.K. Leung, and C.D. MacCalman Expression of ADAMTS-5/implantin in human decidual stromal cells: regulatory effects of cytokines Hum. Reprod., January 1, 2007; 22(1): 63 - 74. [Abstract] [Full Text] [PDF]

				Y. H. Ng, H. Zhu, C. J. Pallen, P. C.K. Leung, and C. D. MacCalman Differential effects of interleukin-1beta and transforming growth factor-beta1 on the expression of the inflammation-associated protein, ADAMTS-1, in human decidual stromal cells in vitro Hum. Reprod., August 1, 2006; 21(8): 1990 - 1999. [Abstract] [Full Text] [PDF]

				S Abdul-Rasool, S H Kidson, E Panieri, D Dent, K Pillay, and G S Hanekom An evaluation of molecular markers for improved detection of breast cancer metastases in sentinel nodes. J. Clin. Pathol., March 1, 2006; 59(3): 289 - 297. [Abstract] [Full Text] [PDF]

				C. Bruse, D. Radu, and A. Bergqvist In situ localization of mRNA for the fibrinolytic factors uPA, PAI-1 and uPAR in endometriotic and endometrial tissue Mol. Hum. Reprod., March 1, 2004; 10(3): 159 - 166. [Abstract] [Full Text] [PDF]

				C.-S. Chou, C.-J. Tai, C. D. MacCalman, and P. C. K. Leung Dose-Dependent Effects of Gonadotropin Releasing Hormone on Matrix Metalloproteinase (MMP)-2, and MMP-9 and Tissue Specific Inhibitor of Metalloproteinases-1 Messenger Ribonucleic Acid Levels in Human Decidual Stromal Cells in Vitro J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 680 - 688. [Abstract] [Full Text] [PDF]