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Age-Related Differences in Messenger Ribonucleic Acid Expression of Key Proteins Involved in Adipose Cell Differentiation and Metabolism¹

Physical Activity Sciences Laboratory, Department of Social and Preventive Medicine, Laval University (P.I., A.T., P.M.), Ste-Foy, Québec, Canada G1K 7P4; INSERM U-449, Faculté de Médecine R.T.H. Laënnec (H.V., N.V.), F-69372 Lyon, France; Diabetes Research Unit (A.N.), and Lipid Research Center (J.-P.D., P.M.), Laval Medical Research Center, Québec, Canada G1V 4G2; and Quebec Heart Institute, Laval Hospital (J.-P.D.), Québec, Canada G1V 4G5

Address all correspondence and requests for reprints to: P. Mauriège, Ph.D., Lipid Research Center, Laval University Medical Research Center, 2705 boulevard Laurier, Room TR-93, Ste-Foy, Québec, Canada G1V 4G2. E-mail: mauriege{at}club-internet.fr

This study was performed to compare the expression of key proteins [lipoprotein lipase (LPL), hormone-sensitive lipase (HSL), complement 3 (C3), and peroxisome proliferator-stimulated receptor- (PPAR)] involved in sc abdominal adipose tissue (AT) metabolism of young (n = 13) vs. middle-aged (n = 16) men. The sc abdominal AT-LPL activity as well as fat cell lipolysis were also measured in both groups of men. Young and middle-aged men displayed similar body weight and sc abdominal fat accumulation, measured by computed tomography. However, middle-aged men were characterized by a higher percent body fat (28 ± 5% vs. 22 ± 7%; P < 0.05) than young subjects. No difference between groups was observed in sc abdominal adipose tissue LPL activity. On the other hand, maximal lipolytic responses of sc abdominal adipocytes to isoproterenol (ß-adrenergic agonist) or to postadrenoceptor agents such as dibutyryl cAMP, forskolin, and theophylline were lower in middle-aged than in young men (P < 0.05). AT-LPL messenger ribonucleic acid (mRNA) levels were similar regardless of the subject’s age. However, HSL, C3, and PPAR mRNA levels were higher in middle-aged than in young individuals (P < 0.01–0.05). After correction for percent body fat, only HSL and C3 mRNA levels remained significantly different between groups (P < 0.05). Taken together, these results suggest that aging has an effect on the up-regulation of HSL and C3 mRNA levels, whereas PPAR expression seems to be related mainly to increased adiposity.

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