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Placental Transport of Leucine, Phenylalanine, Glycine, and Proline in Intrauterine Growth-Restricted Pregnancies

Department of Obstetrics and Gynecology (C.L.P., A.M.M., S.R., M.D.N., P.V.F., G.P., F.C.B.), San Paolo Department of Medicine Surgery and Dentistry, University of Milan, 20142 Milan, Italy; and Division of Perinatal Medicine, University of Colorado School of Medicine (P.V.F., F.C.B.), Denver, Colorado 80262

Address all correspondence and requests for reprints to: Anna Maria Marconi, M.D., Department of Obstetrics and Gynecology, San Paolo Department of Medicine, University of Milan, Via A. di Rudini 8, 20142 Milan, Italy. E-mail: marconi{at}enter.it

Abstract

L-[1-¹³C]Leucine, [1-¹³C]glycine, L-[1-¹³C]phenylalanine, and L-[1-¹³C]proline were infused as a bolus into the maternal circulation of seven appropriate for gestational age at 30.3 ± 3.0 wk and 7 intrauterine growth-restricted pregnancies at 26.5 ± 1.0 wk gestation to investigate placental transport in vivo. Umbilical venous samples were obtained at the time of in utero fetal blood sampling at 450 ± 74 sec from the bolus injection. In normal pregnancies the fetal/maternal (F/M) enrichment ratios for leucine (0.76 ± 0.06) and phenylalanine (0.77 ± 0.06) were higher (P < 0.01) than the F/M ratios for glycine (0.18 ± 0.04) and proline (0.22 ± 0.02). This suggests that these two essential amino acids rapidly cross the placenta in vivo. Compared with the essentials, both glycine and proline had significantly lower F/M enrichment ratios, which were not different from each other. The results support the hypothesis that amino acids with high affinity for exchange transporters cross the placenta most rapidly. In intrauterine growth-restricted pregnancies, the F/M enrichment ratio was significantly lower (P < 0.01) for L-[1-¹³C]leucine (0.76 ± 0.06 vs. 0.48 ± 0.07) and for L-[1-¹³C]phenylalanine (0.77 ± 0.06 vs. 0.46 ± 0.07) compared with appropriate for gestational age pregnancies reflecting impaired transplacental flux. The F/M enrichment ratio did not differ for [1-¹³C]glycine (0.18 ± 0.04 vs. 0.17 ± 0.03), and L-[1-¹³C]proline (0.22 ± 0.02 vs. 0.18 ± 0.04).

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