This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Magnan, C. Articles by Ktorza, A. Search for Related Content PubMed PubMed Citation Articles by Magnan, C. Articles by Ktorza, A. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB GLUCOSE

Glucose-Induced Insulin Hypersecretion in Lipid-Infused Healthy Subjects Is Associated with a Decrease in Plasma Norepinephrine Concentration and Urinary Excretion

Université Paris 7 Centre National de la Recherche Scientifique ESA 7059 (C.M., C.C., L.C., M.V., A.K.), 75251 Paris cedex 05, France; Groupe Hospitalier Necker-Enfants-Malades (INSERM CIC 9303) (N.B., J.-L.B.), Laboratoire de Pharmacologie-Néphrologie (A.G., J.-L.E.), Hôpital Necker, INSERM U 342 (G.V.), Hôpital St Vincent de Paul, 75743 Paris Cedex 15, France; Lipha Merck (P.A., M.K.), 91475 Chilly-Mazarin Chilly-Mazarin, France

Address all correspondence and requests for reprints to: Christophe Magnan, Université Paris 7, Centre National de la Recherche Scientifique ESA 7059, Case 7126, 2 place Jussieu, 75251 Paris cedex 05, France. E-mail: magnan{at}paris7.jussieu.fr

Abstract

We investigated the effect of a 48 h triglyceride infusion on the subsequent insulin secretion in response to glucose in healthy men. We measured the variations in plasma concentration and urinary excretion of catecholamines as an indirect estimation of sympathetic tone. For 48 h, 20 volunteers received a triglyceride/heparin or a saline solution, separated by a 1-month interval. At time 48 h, insulin secretion in response to glucose was investigated by a single iv glucose injection (0.5 g/kg^-1) followed by an hyperglycemic clamp (10 mg·kg^-1·min^-1, during 50 min). The triglyceride infusion resulted in a 3-fold elevation in plasma free fatty acids and an increase in insulin and C-peptide plasma concentrations (1.5- and 2.5-fold, respectively, P < 0.05), compared with saline. At time 48 h of lipid infusion, plasma norepinephrine (NE) concentration and urinary excretion levels were lowered compared with saline (plasma NE: 0.65 ± 0.08 vs. 0.42 ± 0.06 ng/ml, P < 0.05; urinary excretion: 800 ± 70 vs. 620 ± 25 nmol/24 h, P < 0.05). In response to glucose loading, insulin and C-peptide plasma concentrations were higher in lipid compared with saline infusion (plasma insulin: 600 ± 98 vs. 310 ± 45 pM, P < 0.05; plasma C-peptide 3.5 ± 0.2 vs. 1.7 ± 0.2 nM, P < 0.05). In conclusion, in healthy subjects, a 48-h lipid infusion induces basal hyperinsulinemia and exaggerated insulin secretion in response to glucose which may be partly related to a decrease in sympathetic tone.

This article has been cited by other articles:

				C. S. Katsanos, A. Aarsland, M. G. Cree, and R. R. Wolfe Muscle Protein Synthesis and Balance Responsiveness to Essential Amino Acids Ingestion in the Presence of Elevated Plasma Free Fatty Acid Concentrations J. Clin. Endocrinol. Metab., August 1, 2009; 94(8): 2984 - 2990. [Abstract] [Full Text] [PDF]

				A. I. Oprescu, G. Bikopoulos, A. Naassan, E. M. Allister, C. Tang, E. Park, H. Uchino, G. F. Lewis, I. G. Fantus, M. Rozakis-Adcock, et al. Free Fatty Acid Induced Reduction in Glucose-Stimulated Insulin Secretion: Evidence for a Role of Oxidative Stress In Vitro and In Vivo Diabetes, December 1, 2007; 56(12): 2927 - 2937. [Abstract] [Full Text] [PDF]

				K. D. Monahan, D. J. Dyckman, and C. A. Ray Effect of acute hyperlipidemia on autonomic and cardiovascular control in humans J Appl Physiol, July 1, 2007; 103(1): 162 - 169. [Abstract] [Full Text] [PDF]

				K. Cusi, S. Kashyap, A. Gastaldelli, M. Bajaj, and E. Cersosimo Effects on insulin secretion and insulin action of a 48-h reduction of plasma free fatty acids with acipimox in nondiabetic subjects genetically predisposed to type 2 diabetes Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1775 - E1781. [Abstract] [Full Text] [PDF]

				T. T. Goh, T. M. Mason, N. Gupta, A. So, T. K. T. Lam, L. Lam, G. F. Lewis, A. Mari, and A. Giacca Lipid-induced beta-cell dysfunction in vivo in models of progressive beta-cell failure Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E549 - E560. [Abstract] [Full Text] [PDF]

				S. Migrenne, C. Cruciani-Guglielmacci, L. Kang, R. Wang, C. Rouch, A.-L. Lefevre, A. Ktorza, V. H. Routh, B. E. Levin, and C. Magnan Fatty Acid Signaling in the Hypothalamus and the Neural Control of Insulin Secretion Diabetes, December 1, 2006; 55(Supplement_2): S139 - S144. [Abstract] [Full Text] [PDF]

				S. Kashyap, R. Belfort, A. Gastaldelli, T. Pratipanawatr, R. Berria, W. Pratipanawatr, M. Bajaj, L. Mandarino, R. DeFronzo, and K. Cusi A Sustained Increase in Plasma Free Fatty Acids Impairs Insulin Secretion in Nondiabetic Subjects Genetically Predisposed to Develop Type 2 Diabetes Diabetes, October 1, 2003; 52(10): 2461 - 2474. [Abstract] [Full Text] [PDF]