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Deficiency of Total and Nonglycosylated Amylin in Plasma Characterizes Subjects with Impaired Glucose Tolerance and Type 2 Diabetes¹

Department of Medicine, Division of Diabetes (S.M., H.Y.-J.), University of Helsinki, 00029 HUCH, Helsinki, Finland; and Amylin Pharmaceuticals, Inc. (M.S.F.), San Diego, California 92121

Address correspondence and requests for reprints to: Hannele Yki-Järvinen, M.D., Department of Medicine, Division of Diabetes, University of Helsinki, P.O. Box 340, 00029 HUCH, Helsinki, Finland. E-mail: ykijarvi{at}helsinki.fi

This study was undertaken to characterize first and second phase secretory profiles of total and nonglycosylated amylin and insulin and to determine whether excessive glycosylation of amylin or hyperamylinemia is a feature of abnormal glucose tolerance in humans. Plasma concentrations of total and nonglycosylated amylin and serum immunoreactive insulin were measured under identical hyperglycemic conditions using the hyperglycemic clamp technique in subjects with type 2 diabetes, impaired and normal glucose tolerance. Both amylin and insulin concentrations followed a biphasic pattern in subjects with normal and impaired glucose tolerance. In the subjects with normal and impaired glucose tolerance, the second phase amylin concentrations markedly exceeded those of the first phase, whereas the reverse was true for insulin. The first phase concentrations of both peptides were significantly lower in impaired than the normal glucose tolerance subjects. In patients with type 2 diabetes no first phase peak for either amylin or insulin could be identified, and the second phases of both amylin and insulin were significantly lower compared to subjects with normal or impaired glucose tolerance. Nonglycosylated amylin concentrations accounted for 25–45% of total amylin, regardless of glucose tolerance, and mimicked the pattern of total amylin concentrations. In summary: 1) glucose-induced increases in the magnitude of the first and second phase amylin plasma concentrations differed from those of insulin; 2) subjects with impaired glucose tolerance and more strikingly those with type 2 diabetes have impaired amylin responses; and 3) the ratio of nonglycosylated to total amylin is normal irrespective of glucose tolerance. These data imply, in view of many reports describing accumulation of amyloid in the pancreas, that circulating levels of amylin decrease as amyloid deposits accumulate and ß-cell function deteriorates and that the amount of glycosylated amylin in plasma is not increased in patients with type 2 diabetes.

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