This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Purchase Article View Shopping Cart Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Speirs, V. Articles by Atkin, S. L. Search for Related Content PubMed PubMed Citation Articles by Speirs, V. Articles by Atkin, S. L.

Identification of Wild-Type and Exon 5 Deletion Variants of Estrogen Receptor ß in Normal Human Mammary Gland¹

Medical Research Laboratory, Wolfson Building, University of Hull, Hull HU6 7RX, United Kingdom

Address correspondence and requests for reprints to: Dr. Valerie Speirs, Molecular Medicine Unit, Clinical Sciences Building, St. James’s University Hospital, Leeds LS9 7TF, United Kindgom. E-mail: v.speirs{at}leeds.ac.uk

We have examined messenger RNA (mRNA) expression of estrogen receptor (ER) , wild-type ERß (mRNA and protein), and ERß exon 5 deletion variants (ERß 5) in samples of normal human mammary gland obtained from 37 premenopausal subjects undergoing reduction mammoplasty. Comparing individual expression, ERß mRNA predominated, expressed in 34 of 37 samples (91%), whereas ER was found in 21 of 37 cases (57%). Receptor combinations were then analyzed and compared. Most samples either coexpressed ER with ERß (54%) or expressed just ERß (38%). Immunohistochemical analysis revealed that ERß mRNA expression mirrored that of protein. Immunoreactivity was observed in the nucleus with additional evidence of cytoplasmic staining in those epithelial cells lining the breast ducts. Sporadic immunoreactivity was also detected in stromal cells. Expression of wild type and ERß 5 was analyzed, and their association with ER was compared. Most samples coexpressed wild-type ERß and the splice variant (62%; P = 0.05), with 30% exclusively expressing wild-type ERß. Although samples coexpressing wild type and variant ERß showed no statistical association with ER, those samples expressing only wild-type ERß, showed a trend toward associations with ER (P = 0.07). In conclusion, our data would support a role for ERß in the normal human mammary gland, where we propose it may be the dominant receptor.

This article has been cited by other articles:

				O. Treeck, G. Pfeiler, D. Mitter, C. Lattrich, G. Piendl, and O. Ortmann Estrogen receptor {beta}1 exerts antitumoral effects on SK-OV-3 ovarian cancer cells J. Endocrinol., June 1, 2007; 193(3): 421 - 433. [Abstract] [Full Text] [PDF]

				U. Ohnemus, M. Uenalan, J. Inzunza, J.-A. Gustafsson, and R. Paus The Hair Follicle as an Estrogen Target and Source Endocr. Rev., October 1, 2006; 27(6): 677 - 706. [Abstract] [Full Text] [PDF]

				J-R Long, H Xu, L-J Zhao, P-Y Liu, H Shen, Y-J Liu, D-H Xiong, P Xiao, Y-Z Liu, V Dvornyk, et al. The oestrogen receptor {alpha} gene is linked and/or associated with age of menarche in different ethnic groups J. Med. Genet., October 1, 2005; 42(10): 796 - 800. [Full Text] [PDF]

				C. Palmieri, E. W.-F. Lam, J. Mansi, C. MacDonald, S. Shousha, P. Madden, Y. Omoto, A. Sunters, M. Warner, J.-A. Gustafsson, et al. The Expression of ER{beta}cx in Human Breast Cancer and the Relationship to Endocrine Therapy and Survival Clin. Cancer Res., April 1, 2004; 10(7): 2421 - 2428. [Abstract] [Full Text] [PDF]

				S. L. Zheng, W. Zheng, B.-l. Chang, X.-O. Shu, Q. Cai, H. Yu, Q. Dai, J. Xu, and Y.-T. Gao Joint Effect of Estrogen Receptor {beta} Sequence Variants and Endogenous Estrogen Exposure on Breast Cancer Risk in Chinese Women Cancer Res., November 15, 2003; 63(22): 7624 - 7629. [Abstract] [Full Text] [PDF]

				L. Xu, Q. Pan-Hammarstrom, A. Forsti, K. Hemminki, L. Hammarstrom, D. Labuda, J.-A. Gustafsson, and K. Dahlman-Wright Human Estrogen Receptor {beta} 548 Is Not a Common Variant in Three Distinct Populations Endocrinology, August 1, 2003; 144(8): 3541 - 3546. [Abstract] [Full Text] [PDF]

				V Speirs, G P Skliris, S E Burdall, and P J Carder Distinct expression patterns of ER{alpha} and ER{beta} in normal human mammary gland J. Clin. Pathol., May 1, 2002; 55(5): 371 - 374. [Abstract] [Full Text] [PDF]

				H. A. LaVoie, D. C. DeSimone, C. Gillio-Meina, and Y. Y. Hui Cloning and Characterization of Porcine Ovarian Estrogen Receptor {beta} Isoforms Biol Reprod, March 1, 2002; 66(3): 616 - 623. [Abstract] [Full Text] [PDF]

				S. Saji, H. Sakaguchi, S. Andersson, M. Warner, and J.-A. Gustafsson Quantitative Analysis of Estrogen Receptor Proteins in Rat Mammary Gland Endocrinology, July 1, 2001; 142(7): 3177 - 3186. [Abstract] [Full Text] [PDF]

				H. Cardenas, K.A. Burke, R.M. Bigsby, W.F. Pope, and K.P. Nephew Estrogen Receptor {beta} in the Sheep Ovary During the Estrous Cycle and Early Pregnancy Biol Reprod, July 1, 2001; 65(1): 128 - 134. [Abstract] [Full Text] [PDF]