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Expression of Oncofetal Fibronectin Messenger Ribonucleic Acid in Fibroblasts in the Thyroid: A Possible Cause of False Positive Results in Molecular-Based Diagnosis of Thyroid Carcinomas¹

Department of Laboratory Medicine, Osaka University Medical School (T.T., N.A.), D2, 2–2 Yamadaoka, Suita, Osaka 565-0871; and Kuma Hospital (A.M., F.M., K.K.), 8–2-35, Simoyamate-Dori, Chuo-Ku, Kobe, Hyogo 650-0011, Japan

Address all correspondence and requests for reprints to: Dr. Toru Takano, Department of Laboratory Medicine, D2, Osaka University Medical School, 2–2 Yamadaoka, Suita, Osaka 565-0871, Japan.

Oncofetal fibronectin (onfFN) messenger ribonucleic acid (mRNA) is abundantly expressed in thyroid papillary and anaplastic carcinomas. These carcinomas can be preoperatively diagnosed by the detection of onfFN mRNA in fine needle aspiration biopsies (FNABs). However, previous reports have noted that the expression of onfFN mRNA was observed in 3.7% of the FNABs that were diagnosed as negative cytology. To clarify this discrepancy, we examined the expression of onfFN mRNA in fibroblasts in the thyroid. By RT-PCR and real-time quantitative RT-PCR analyses, we detected a high copy number of onfFN mRNA in cultured fibroblasts obtained from the normal thyroid tissues dissected surgically. Thus, we conclude that the contaminated fibroblasts in FNABs due to tumor necrosis or acute or chronic inflammation may be a cause of false positive results in molecular-based diagnosis of thyroid carcinomas.

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