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Interleukin-1 Receptor Antagonist Ribonucleic Acid and Protein Expression by Cultured Graves’ and Normal Orbital Fibroblasts Is Differentially Modulated by Dexamethasone and Irradiation¹

Division of Endocrinology, Philipps University (W.J., C.S., A.E.H.), D-35033 Marburg; University Eye Hospital, Klinikum Innenstadt, Ludwig Maximilians Universität (H.-D.S.), D-80336 Munich; and Städtisches Klinikum Leipzig-West (T.M., H.-J.H.), D-04177 Leipzig, Germany

Address all correspondence and requests for reprints to: Armin E. Heufelder, M.D., Division of Gastroenterology, Endocrinology and Metabolism, Department of Internal Medicine, Philipps University, Baldingerstrasse, D-35033 Marburg, Germany. E-mail: heufeld{at}mailer.uni-marburg.de

Recent data have indicated that orbital fibroblasts (OF) can be stimulated to produce marked quantities of interleukin-1 receptor antagonist (IL-1RA), a powerful inhibitor of the proinflammatory activities of interleukin-1 in the orbital tissues in Graves’ ophthalmopathy (GO). We examined whether the beneficial effects of dexamethasone or irradiation, the two main therapeutic modalities applied in patients with active GO, may be related to their capacity to alter IL-1RA ribonucleic acid (RNA) and protein expression in OF. Early passages of cultured OF were obtained from orbital connective tissue and extraocular muscle of patients with severe active GO and five control subjects. Modulation of the two variants of IL-1RA, intracellular IL-1RA (icIL-1RA) and soluble IL-1RA (sIL-1RA), was studied after exposure of OF to increasing concentrations of dexamethasone (10^-10-10^-6 mol/L), the glucocorticoid receptor antagonist RU 38486 (10^-3 mol/L), or combinations thereof. Alternatively, cell monolayers were exposed to increasing doses of UV irradiation (0.1–1 J/cm²) or ionizing irradiation (0.2–2 Gy). The IL-1RA gene and protein variants were analyzed by RT-PCR, immunocytochemistry, immunoblotting, and enzyme-linked immunosorbent assay. Dexamethasone inhibited IL-1RA RNA steady state levels in GO OF and control OF in a dose-dependent manner. Combined exposure of OF to dexamethasone and RU 38486 completely restored baseline levels of IL-1RA RNA. By contrast, low doses of UV and ionizing irradiation dose dependently up-regulated IL-1RA-specific transcripts in GO OF and control OF, whereas higher doses were less effective. Immunoblotting and enzyme-linked immunosorbent assay revealed suppression of IL-1RA immunoreactivity after treatment with dexamethasone and enhanced expression of IL-1RA by GO OF and normal OF after low doses of UV and ionizing irradiation. Our results indicate that, in contrast to dexamethasone, low doses of irradiation stimulate expression of the IL-1RA gene and protein variants in OF. Induction by irradiation of IL-1RA expression in target cells of the orbital immune process represents an as yet unrecognized mechanism by which orbital radiotherapy may exert some of its beneficial therapeutic effects in patients with active GO.

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