Gonadotropin- and Cytokine-Regulated Expression of the Chemokine Interleukin 8 in the Human Preovulatory Follicle of the Menstrual Cycle1
Eva Runesson,
Karin Ivarsson,
Per Olof Janson and
Mats Brännström
Department of Obstetrics and Gynecology, Institute for the Health
of Women and Children, Göteborg University, 413 45
Göteborg, Sweden
Address correspondence and requests for reprints to: Eva Runesson, Department of Obstetrics and Gynecology, Göteborg University, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden. E-mail:
eva.runesson{at}medfak.gu.se
Interleukin 8 (IL-8) is a chemotactic cytokine involved in the
recruitmentand activation of neutrophils as well as in cell
proliferationand angiogenesis. Because these events are essential
componentsof folliculogenesis, ovulation, and subsequent repair of the
rupturedfollicle, the presence and regulation of IL-8 in the human
follicleof the menstrual cycle was investigated. The concentrationsof
IL-8 were higher in follicular fluids from dominant folliclesof late
follicular/ovulatory phase compared with those of midfollicularphase.
IL-8 was detected in the media from cultured granulosaand theca cells,
with 10-fold higher levels in the theca cellcultures. Exposure to FSH
and LH increased the IL-8 secretionfrom granulosa cells, but no effect
was seen in theca cell cultures.Estradiol and progesterone did not
affect IL-8 secretion fromany cell type. The cytokines IL-1 and
IL-1ß, but not tumornecrosis factor , enhanced IL-8 secretion
from both cell types.IL-8 levels in cultures of granulosa-lutein cells
from hyperstimulatedin vitro fertilization
cycles were not affected by either gonadotropinsor steroids. These
data provide evidence that ovarian IL-8 isgonadotropin and cytokine
induced and may be involved in thehormonally regulated stages of
follicular development and ovulation.
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and
IL-1ß, but not tumor necrosis factor 
