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The Journal of Clinical Endocrinology & Metabolism Vol. 85, No. 11 4293-4297
Copyright © 2000 by The Endocrine Society

Original Studies

Expression of Genes Involved in Lipid Metabolism Correlate with Peroxisome Proliferator-Activated Receptor {gamma} Expression in Human Skeletal Muscle1

Naras M. Lapsys2, Adamandia D. Kriketos2, Megan Lim-Fraser, Ann M. Poynten, Andrew Lowy, Stuart M. Furler, Donald J. Chisholm and Gregory J. Cooney

Metabolism and Diabetes Research Group, The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia

Address all correspondence and requests for reprints to: Dr. Naras Lapsys, Metabolism and Diabetes Research Group, The Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia. E-mail: n.lapsys{at}garvan.unsw.edu.au

Peroxisome proliferator-activated receptor {gamma} (PPAR-{gamma}) activation in adipose tissue is known to regulate genes involved in adipocyte differentiation and lipid metabolism. However, the role of PPAR-{gamma} in muscle remains unclear. To examine the potential regulation of genes by PPAR-{gamma} in human skeletal muscle, we used semiquantitative RT-PCR to determine the expression of PPAR-{gamma}, lipoprotein lipase (LPL), muscle carnitine palmitoyl transferase-1 (mCPT1), fatty acid-binding protein (FABP), carnitine acylcarnitine transferase (CACT), and glucose transporter-4 (GLUT4) in freeze-dried muscle samples from 14 male subjects. These samples were dissected free of adipose and other tissue contamination, as confirmed by minimal or absent adipsin expression. Between individuals, the messenger ribonucleic acid concentration of PPAR-{gamma} varied up to 3-fold, whereas LPL varied up to 6.5-fold, mCPT1 13-fold, FABP 4-fold, CACT 4-fold, and GLUT4 up to 3-fold. The expression of LPL (r2 = 0.54; P = 0.003), mCPT1 (r2 = 0.42; P = 0.012), and FABP (r2 = 0.324; P = 0.034) all correlated significantly with PPAR-{gamma} expression in the same samples. No significant correlation was observed between the expression of CACT and PPAR-{gamma} or between GLUT4 and PPAR-{gamma}. These findings demonstrate a relationship between PPAR-{gamma} expression and the expression of other genes of lipid metabolism in muscle and support the hypothesis that PPAR-{gamma} activators such as the antidiabetic thiazolidinediones may regulate fatty acid metabolism in skeletal muscle as well as in adipose tissue.




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