help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Demeterco, C.
Right arrow Articles by Hayek, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demeterco, C.
Right arrow Articles by Hayek, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
The Journal of Clinical Endocrinology & Metabolism Vol. 85, No. 10 3892-3897
Copyright © 2000 by The Endocrine Society

Original Studies

A Role for Activin A and Betacellulin in Human Fetal Pancreatic Cell Differentiation and Growth1

Carla Demeterco, Gillian M. Beattie, Sergio Atala Dib, Ana D. Lopez and Alberto Hayek

Whittier Institute and Department of Pediatrics (C.D., G.M.B., A.D.L., A.H.), University of California at San Diego, La Jolla, California 92037; and Federal University of Sao Paulo (C.D., S.A.D.), Sao Paulo, Brazil

Address correspondence and requests for reprints to: Dr. Alberto Hayek, The Islet Research Laboratory, Department of Pediatrics, University of California at San Diego, 9894 Genesee Avenue, La Jolla, California 92037. E-mail: ahayek{at}ucsd.edu

Activin A (Act.A), a member of the transforming growth factor ß family of secreted proteins, has been implicated in the regulation of growth and differentiation of various cell types. Betacellulin (BTC), a member of the epidermal growth factor family, converts exocrine AR42J cells to insulin-expressing cells when combined with Act.A. We have used primary cultures of human fetal pancreatic tissue to identify the effects of Act.A and/or BTC on islet development and growth. Exposure to Act.A resulted in a 1.5-fold increase in insulin content (P < 0.005) and a 2-fold increase in the number of cells immunopositive for insulin (P < 0.005). The formation of islet-like cell clusters, containing mainly epithelial cells, during a 5-day culture, was stimulated 1.4-fold by BTC (P < 0.05). BTC alone caused a 2.6-fold increase in DNA synthesis (P < 0.005). These data suggest that Act.A induces endocrine differentiation, whereas BTC has a mitogenic effect on human undifferentiated pancreatic epithelial cells.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. L. Schneyer, Y. Sidis, A. Gulati, J. L. Sun, H. Keutmann, and P. A. Krasney
Differential Antagonism of Activin, Myostatin and Growth and Differentiation Factor 11 by Wild-Type and Mutant Follistatin
Endocrinology, September 1, 2008; 149(9): 4589 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
T. Brun and B. R Gauthier
A focus on the role of Pax4 in mature pancreatic islet {beta}-cell expansion and survival in health and disease
J. Mol. Endocrinol., February 1, 2008; 40(2): 37 - 45.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. Jiang, M. Au, K. Lu, A. Eshpeter, G. Korbutt, G. Fisk, and A. S. Majumdar
Generation of Insulin-Producing Islet-Like Clusters from Human Embryonic Stem Cells
Stem Cells, August 1, 2007; 25(8): 1940 - 1953.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. Gromada, I. Franklin, and C. B. Wollheim
{alpha}-Cells of the Endocrine Pancreas: 35 Years of Research but the Enigma Remains
Endocr. Rev., February 1, 2007; 28(1): 84 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Mamin and J. Philippe
Activin A Decreases glucagon and arx Gene Expression in {alpha}-Cell Lines
Mol. Endocrinol., January 1, 2007; 21(1): 259 - 273.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Bertelli and M. Bendayan
Association between Endocrine Pancreas and Ductal System. More than an Epiphenomenon of Endocrine Differentiation and Development?
J. Histochem. Cytochem., September 1, 2005; 53(9): 1071 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Zalzman, L. Anker-Kitai, and S. Efrat
Differentiation of Human Liver-Derived, Insulin-Producing Cells Toward the {beta}-Cell Phenotype
Diabetes, September 1, 2005; 54(9): 2568 - 2575.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. Silver, M. Tolea, J. Wang, T. I. Pollin, F. Yao, and B. D. Mitchell
The Exon 1 Cys7Gly Polymorphism Within the Betacellulin Gene Is Associated With Type 2 Diabetes in African Americans
Diabetes, April 1, 2005; 54(4): 1179 - 1184.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G. M. Beattie, A. D. Lopez, N. Bucay, A. Hinton, M. T. Firpo, C. C. King, and A. Hayek
Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers
Stem Cells, April 1, 2005; 23(4): 489 - 495.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Brun, I. Franklin, L. St-Onge, A. Biason-Lauber, E. J. Schoenle, C. B. Wollheim, and B. R. Gauthier
The diabetes-linked transcription factor PAX4 promotes {beta}-cell proliferation and survival in rat and human islets
J. Cell Biol., December 20, 2004; 167(6): 1123 - 1135.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y.-Q. Zhang, M. M. Cleary, Y. Si, G. Liu, Y. Eto, M. Kritzik, S. Dabernat, A. G. Kayali, and N. Sarvetnick
Inhibition of Activin Signaling Induces Pancreatic Epithelial Cell Expansion and Diminishes Terminal Differentiation of Pancreatic {beta}-Cells
Diabetes, August 1, 2004; 53(8): 2024 - 2033.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Li, Z. Yi, M. Seno, and I. Kojima
Activin A and Betacellulin: Effect on Regeneration of Pancreatic {beta}-Cells in Neonatal Streptozotocin-Treated Rats
Diabetes, March 1, 2004; 53(3): 608 - 615.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. K. Humphrey, N. Bucay, G. M. Beattie, A. Lopez, C. A. Messam, V. Cirulli, and A. Hayek
Characterization and Isolation of Promoter-Defined Nestin-Positive Cells from the Human Fetal Pancreas
Diabetes, October 1, 2003; 52(10): 2519 - 2525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Li, M. Seno, H. Yamada, and I. Kojima
Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells to {beta}-cells in streptozotocin-treated mice
Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E577 - E583.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. Movassat, G. M. Beattie, A. D. Lopez, and A. Hayek
Exendin 4 Up-Regulates Expression of PDX 1 and Hastens Differentiation and Maturation of Human Fetal Pancreatic Cells
J. Clin. Endocrinol. Metab., October 1, 2002; 87(10): 4775 - 4781.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-Q. Zhang, H. Zhang, A. Maeshima, H. Kurihara, J.-i. Miyagawa, T. Takeuchi, and I. Kojima
Up-Regulation of the Expression of Activins in the Pancreatic Duct by Reduction of the {beta}-Cell Mass
Endocrinology, September 1, 2002; 143(9): 3540 - 3547.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
C. Demeterco, P. Itkin-Ansari, B. Tyrberg, L. P. Ford, R. A. Jarvis, and F. Levine
c-Myc Controls Proliferation Versus Differentiation in Human Pancreatic Endocrine Cells
J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3475 - 3485.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Li, M. Seno, H. Yamada, and I. Kojima
Promotion of {beta}-Cell Regeneration by Betacellulin in Ninety Percent-Pancreatectomized Rats
Endocrinology, December 1, 2001; 142(12): 5379 - 5385.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. Dufayet de la Tour, T. Halvorsen, C. Demeterco, B. Tyrberg, P. Itkin-Ansari, M. Loy, S.-J. Yoo, E. Hao, S. Bossie, and F. Levine
{beta}-Cell Differentiation from a Human Pancreatic Cell Line in Vitro and in Vivo
Mol. Endocrinol., March 1, 2001; 15(3): 476 - 483.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society