help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frendo, J. L.
Right arrow Articles by Evain-Brion, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frendo, J. L.
Right arrow Articles by Evain-Brion, D.
The Journal of Clinical Endocrinology & Metabolism Vol. 85, No. 10 3700-3707
Copyright © 2000 by The Endocrine Society

Original Studies

Defect of Villous Cytotrophoblast Differentiation into Syncytiotrophoblast in Down’s Syndrome1

J. L. Frendo, M. Vidaud, J. Guibourdenche, D. Luton, F. Muller, D. Bellet, Y. Giovagrandi, A. Tarrade, D. Porquet, P. Blot and D. Evain-Brion

INSERM, U-427 (J.L.F., A.T., D.B.-E.), Laboratoire de Génétique Moléculaire (M.V., Y.G.), Centre National de la Recherche Scientifique (D.B.), UPRES-A 8067, Faculté des Sciences Pharmaceutiques et Biologiques, Université René Descartes, 75270 Paris, France; Service d’Hormonologie (J.G., D.P.) and Service de Gynécologie Obstétrique (D.L., P.B.), Hôpital Robert Debré, 75019 Paris, France; and Service de Biochimie, Hôpital Ambroise Paré (F.M.), 92104 Boulogne, France

Address all correspondence and requests for reprints to: Dr. D. Evain-Brion, INSERM U-427, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l’Observatoire, 75270 Paris Cedex 06, France. E-mail: evain{at}pharmacie.univ-paris5.fr

The syncytiotrophoblast (ST) is one of the major components of the human placenta, as it is involved in feto-maternal exchanges and the secretion of pregnancy-specific hormones. The aim of this study was to elucidate the formation and function of the ST in trisomy 21 (Down’s syndrome). We first used the in vitro model of cytotrophoblast differentiation into ST. Cytotrophoblasts were isolated from 15 trisomy 21-affected placentas (12–35 weeks gestation) and 10 gestational age-matched control placentas. In vitro cytotrophoblasts isolated from normal placenta fused to form the ST. This was associated with an increase in transcript levels and in the secretion of hCG, human placental lactogen, placental GH, and leptin. In trisomy 21-affected placentas, we observed a defect (or a delay) in ST formation and a dramatic decrease in the synthesis and secretion of these hormones compared to those in cultured cells isolated from control age-matched placentas. These results were confirmed by a significant (P < 0.001) decrease in gene expression in total homogenates of trisomy 21-affected placentas compared to controls. These results will be of help in understanding the maternal hormonal markers of fetal trisomy 21 and the consequences of placental defects for fetal development.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
G. Pidoux, P. Gerbaud, O. Marpeau, J. Guibourdenche, F. Ferreira, J. Badet, D. Evain-Brion, and J.-L. Frendo
Human Placental Development Is Impaired by Abnormal Human Chorionic Gonadotropin Signaling in Trisomy 21 Pregnancies
Endocrinology, November 1, 2007; 148(11): 5403 - 5413.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Handschuh, J. Guibourdenche, V. Tsatsaris, M. Guesnon, I. Laurendeau, D. Evain-Brion, and T. Fournier
Human Chorionic Gonadotropin Produced by the Invasive Trophoblast But Not the Villous Trophoblast Promotes Cell Invasion and Is Down-Regulated by Peroxisome Proliferator-Activated Receptor-{gamma}
Endocrinology, October 1, 2007; 148(10): 5011 - 5019.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. J. Roper, H. K. St. John, J. Philip, A. Lawler, and R. H. Reeves
Perinatal Loss of Ts65Dn Down Syndrome Mice
Genetics, January 1, 2006; 172(1): 437 - 443.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. J.N. Weinans, U. Sancken, R. Pandian, J. M.W. van de Ouweland, H. W.A. de Bruijn, J. P. Holm, and A. Mantingh
Invasive Trophoblast Antigen (Hyperglycosylated Human Chorionic Gonadotropin) as a First-Trimester Serum Marker for Down Syndrome
Clin. Chem., July 1, 2005; 51(7): 1276 - 1279.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. T.J.I. Go, A. Visser, M. A.M. Mulders, M. A. Blankenstein, J. M.G. van Vugt, and C. B.M. Oudejans
Detection of Placental Transcription Factor mRNA in Maternal Plasma
Clin. Chem., August 1, 2004; 50(8): 1413 - 1414.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J.-L. Frendo, J. Guibourdenche, G. Pidoux, M. Vidaud, D. Luton, Y. Giovangrandi, D. Porquet, F. Muller, and D. Evain-Brion
Trophoblast Production of a Weakly Bioactive Human Chorionic Gonadotropin in Trisomy 21-Affected Pregnancy
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 727 - 732.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Chellakooty, K. Vangsgaard, T. Larsen, T. Scheike, J. Falck-Larsen, J. Legarth, A. M. Andersson, K. M. Main, N. E. Skakkebaek, and A. Juul
A Longitudinal Study of Intrauterine Growth and the Placental Growth Hormone (GH)-Insulin-Like Growth Factor I Axis in Maternal Circulation: Association between Placental GH and Fetal Growth
J. Clin. Endocrinol. Metab., January 1, 2004; 89(1): 384 - 391.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Blaise, N. de Parseval, L. Benit, and T. Heidmann
Genomewide screening for fusogenic human endogenous retrovirus envelopes identifies syncytin 2, a gene conserved on primate evolution
PNAS, October 28, 2003; 100(22): 13013 - 13018.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J.-L. Frendo, L. Cronier, G. Bertin, J. Guibourdenche, M. Vidaud, D. Evain-Brion, and A. Malassine
Involvement of connexin 43 in human trophoblast cell fusion and differentiation
J. Cell Sci., August 15, 2003; 116(16): 3413 - 3421.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
L. Pavan, A. Tarrade, A. Hermouet, C. Delouis, M. Titeux, M. Vidaud, P. Therond, D. Evain-Brion, and T. Fournier
Human invasive trophoblasts transformed with simian virus 40 provide a new tool to study the role of PPAR{gamma} in cell invasion process
Carcinogenesis, August 1, 2003; 24(8): 1325 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Smallwood, A. Papageorghiou, K. Nicolaides, M.K.R. Alley, A. Jim, G. Nargund, K. Ojha, S. Campbell, and S. Banerjee
Temporal Regulation of the Expression of Syncytin (HERV-W), Maternally Imprinted PEG10, and SGCE in Human Placenta
Biol Reprod, July 1, 2003; 69(1): 286 - 293.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J.-M. Costa, A. Benachi, M. Olivi, Y. Dumez, M. Vidaud, and E. Gautier
Fetal Expressed Gene Analysis in Maternal Blood: A New Tool for Noninvasive Study of the Fetus
Clin. Chem., June 1, 2003; 49(6): 981 - 983.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J.-L. Frendo, D. Olivier, V. Cheynet, J.-L. Blond, O. Bouton, M. Vidaud, M. Rabreau, D. Evain-Brion, and F. Mallet
Direct Involvement of HERV-W Env Glycoprotein in Human Trophoblast Cell Fusion and Differentiation
Mol. Cell. Biol., May 15, 2003; 23(10): 3566 - 3574.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Pandian, J. Lu, and J. Ossolinska-Plewnia
Fully Automated Chemiluminometric Assay for Hyperglycosylated Human Chorionic Gonadotropin (Invasive Trophoblast Antigen)
Clin. Chem., May 1, 2003; 49(5): 808 - 810.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Yang, Z. M. Lei, and Ch. V. Rao
The Central Role of Human Chorionic Gonadotropin in the Formation of Human Placental Syncytium
Endocrinology, March 1, 2003; 144(3): 1108 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
J.-L. FRENDO, P. THEROND, J. GUIBOURDENCHE, M. VIDAUD, and D. EVAIN-BRIONA
Implication of Copper Zinc Superoxide Dismutase (SOD-1) in Human Placenta Development
Ann. N.Y. Acad. Sci., November 1, 2002; 973(1): 297 - 301.
[Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Tarrade, F. Goffin, C. Munaut, R. Lai-Kuen, V. Tricottet, J.-M. Foidart, M. Vidaud, F. Frankenne, and D. Evain-Brion
Effect of Matrigel on Human Extravillous Trophoblasts Differentiation: Modulation of Protease Pattern Gene Expression
Biol Reprod, November 1, 2002; 67(5): 1628 - 1637.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
F. Debieve and K. Thomas
Control of the human inhibin {alpha} chain promoter in cytotrophoblast cells differentiating into syncytium
Mol. Hum. Reprod., March 1, 2002; 8(3): 262 - 270.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Tarrade, K. Schoonjans, L. Pavan, J. Auwerx, C. Rochette-Egly, D. Evain-Brion, and T. Fournier
PPAR{gamma}/RXR{alpha} Heterodimers Control Human Trophoblast Invasion
J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 5017 - 5024.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Tarrade, K. Schoonjans, J. Guibourdenche, J. M. Bidart, M. Vidaud, J. Auwerx, C. Rochette-Egly, and D. Evain-Brion
PPAR{gamma}/RXR{alpha} Heterodimers Are Involved in Human CG{beta} Synthesis and Human Trophoblast Differentiation
Endocrinology, October 1, 2001; 142(10): 4504 - 4514.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
F. Debieve, A. Moiset, K. Thomas, S. Pampfer, and C. Hubinont
Vascular endothelial growth factor and placenta growth factor concentrations in Down's syndrome and control pregnancies
Mol. Hum. Reprod., August 1, 2001; 7(8): 765 - 770.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J.-L. Frendo, P. Therond, T. Bird, N. Massin, F. Muller, J. Guibourdenche, D. Luton, M. Vidaud, W. B. Anderson, and D. Evain-Brion
Overexpression of Copper Zinc Superoxide Dismutase Impairs Human Trophoblast Cell Fusion and Differentiation
Endocrinology, August 1, 2001; 142(8): 3638 - 3648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society