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The Journal of Clinical Endocrinology & Metabolism Vol. 85, No. 10 3582-3588
Copyright © 2000 by The Endocrine Society

Original Studies

Liquid Chromatography-Mass Spectrometry Detection of Corticotropin-Releasing Hormone and Proopiomelanocortin-Derived Peptides in Human Skin1

Andrzej Slominski, Andrzej Szczesniewski and Jacobo Wortsman

Department of Pathology (A.Sl.), University of Tennessee, Memphis, Tennessee 38163; Hitachi Scientific Instruments, Inc. (A.Sz.), Naperville, Illinois; and Department of Internal Medicine (J.W.), Southern Illinois University, Springfield, Illinois 62794

Address correspondence and requests for reprints to: Dr. Andrzej Slominski, Department of Pathology, RM576-BMH Main, University of Tennessee, 899 Madison Avenue, Memphis, Tennessee 38163. E-mail: aslominski{at}utmem.edu

We have previously shown expression of CRH and POMC genes and peptides in the human skin. To ascertain the identity of those peptides, we used methods of peptide extraction and purification combined with the highly specific technique of liquid chromatography-mass spectrometry. Testing extracts of human skin, we identified endogenous peptides with masses and retention times corresponding to CRH, ACTH 1–39, ACTH 1–13, and {alpha}-MSH standards. Thus, conclusive evidence is provided for the presence of CRH and the POMC-derived ACTH 1–39, ACTH 1–13, and {alpha}-MSH peptides in human skin. Direct identification of these peptides is consistent with translation of the corresponding genes, and it also suggests intermediate pituitary lobe-like POMC peptide processing.




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