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Responses of Markers of Bone and Collagen Turnover to Exercise, Growth Hormone (GH) Administration, and GH Withdrawal in Trained Adult Males¹

Metabolic Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital (J.D.W., R.C.C.), 4102 Brisbane, Australia; the Research Center for Endocrinology and Metabolism, Sahlgrenska Hospital (P.A.L., T.R., B.-A.B.), S-41345 Göteborg, Sweden; the Department of Endocrinology, Aarhus Community Hospital (J.O.L.J., J.S.C.), DK-8000 Aarhus C., Denmark; the Department of Endocrinology, Frederico II Hospital (S.L., L.S.), 80131 Napoli, Italy; and the Department of Endocrinology, St. Thomas’s Hospital (N.K., P.H.S.), London, United Kingdom SE1 7EH

Address all correspondence and requests for reprints to: Dr. Jennifer D. Wallace, Metabolic Research Unit, University of Queensland, Department of Medicine, Princess Alexandra Hospital, 4102 Brisbane, Australia. E-mail: jwallace{at}medicine.pa.uq.edu.au

To examine the interactions between acute exercise and GH on markers of bone and collagen turnover and to assess the potential for detecting GH abuse in athletes using these markers, we studied 17 aerobically trained males (age, 26.9 ± 1.5 yr). Sequential studies of exercise, GH administration, and GH withdrawal were undertaken. A randomized, controlled study of rest vs. exercise showed that exercise did not change serum osteocalcin; other markers of formation increased transiently (each P < 0.001): bone-specific alkaline phosphatase (+16.1%), carboxyterminal propeptide of type I procollagen (+14.1%), and procollagen III N-terminal extension peptide (+5.0%). The carboxyterminal cross-linked telopeptide of type I collagen, a bone resorption marker, increased 9.7% (P = 0.018) in response to exercise. A randomized, double blind, placebo-controlled, parallel study of recombinant human GH treatment (0.15 IU/kg·day) for 1 week increased serum osteocalcin (net increase preexercise, +10.0%; P = 0.017), carboxyterminal propeptide of type I procollagen (+17.6%; P = 0.002), procollagen III N-terminal extension peptide (+48.4%; P = 0.001), and carboxyterminal cross-linked telopeptide of type I collagen (53.3%; P = 0.009). Disappearance half-times after cessation of recombinant human GH for pre- and postexercise markers ranged from 248–770 h. We conclude 1) endurance exercise transiently activates bone and collagen turnover; 2) brief GH administration results in similar but quantitatively greater augmentation; and 3) these data will assist in designing a GH detection strategy.

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