help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nilsson, M.
Right arrow Articles by Ericson, L. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nilsson, M.
Right arrow Articles by Ericson, L. E.
The Journal of Clinical Endocrinology & Metabolism Vol. 83, No. 3 945-952
Copyright © 1998 by The Endocrine Society

Original Studies

Cytokines and Thyroid Epithelial Integrity: Interleukin-1{alpha} Induces Dissociation of the Junctional Complex and Paracellular Leakage in Filter-Cultured Human Thyrocytes1

Mikael Nilsson2, Johanna Husmark, Ulla Björkman and Lars E. Ericson

Institute of Anatomy and Cell Biology, Göteborg University, S-413 90 Göteborg, Sweden

Address all correspondence and requests for reprints to: Mikael Nilsson, Institute of Anatomy and Cell Biology, Göteborg University, Box 420, (SE) 405 30, Göteborg, Sweden. E-mail: mikael.olof.nilsson{at}anat.cell.gu.se

Locally produced proinflammatory cytokines are likely to play a pathophysiological role in autoimmune thyroid disease. An important feature of the thyroid, not previously considered in cytokine actions, is the barrier created by the follicular epithelium, which secludes two lumenal autoantigens [thyroglobulin (Tg) and thyroperoxidase] from the extrafollicular space. We examined the influence of recombinant cytokines on the barrier function of human thyrocytes cultured as a tight and polarized monolayer in bicameral chambers. Whereas interleukin (IL)-6 (100 U/mL), interferon-{gamma} (100 U/mL), tumor necrosis factor-{alpha} (10 ng/mL), and transforming growth factor-ß1 (10 ng/mL) had no effects, exposure to IL-1{alpha} for 24–48 h reduced the transepithelial resistance from >1000 to <50 {Omega} x cm2 and increased the paracellular flux of [3H]inulin and exogeneous 125I-Tg. This response to IL-1{alpha}, which was dose dependent (1–1000 U/mL) and reversible, was accompanied by dramatic morphological changes of the epithelial junction complex, including aberrant localization of the tight junction protein zonula occludens-1. At the same time, IL-1{alpha} decreased the apical secretion of endogeneous Tg and stimulated the basolateral release of a novel high-molecular-mass protein. We conclude that IL-1{alpha} reduces the thyroid epithelial barrier without signs of general cytotoxicity. The observation suggests a mechanism by which IL-1{alpha} may promote the exposure of hidden autoantigens to the immune system in thyroid autoimmunity.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
J. S. Tash, R. Chakrasali, S. R. Jakkaraj, J. Hughes, S. K. Smith, K. Hornbaker, L. L. Heckert, S. B. Ozturk, M. K. Hadden, T. G. Kinzy, et al.
Gamendazole, an Orally Active Indazole Carboxylic Acid Male Contraceptive Agent, Targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and Stimulates Il1a Transcription in Rat Sertoli Cells
Biol Reprod, June 1, 2008; 78(6): 1139 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Cheng, W. Cao, C. Fiocchi, J. Behar, P. Biancani, and K. M. Harnett
In vitro model of acute esophagitis in the cat
Am J Physiol Gastrointest Liver Physiol, November 1, 2005; 289(5): G860 - G869.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
V. Estienne, N. Brisbarre, S. Blanchin, J.-M. Durand-Gorde, P. Carayon, and J. Ruf
An in vitro model based on cell monolayers grown on the underside of large- pore filters in bicameral chambers for studying thyrocyte-lymphocyte interactions
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1763 - C1768.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
D. D. Mruk and C. Y. Cheng
Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis
Endocr. Rev., October 1, 2004; 25(5): 747 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W.-y. Lui, W. M. Lee, and C. Y. Cheng
Transforming Growth Factor {beta}3 Regulates the Dynamics of Sertoli Cell Tight Junctions Via the p38 Mitogen-Activated Protein Kinase Pathway
Biol Reprod, May 1, 2003; 68(5): 1597 - 1612.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. W. Slaton, T. Karashima, P. Perrotte, K. Inoue, S. J. Kim, J. Izawa, D. Kedar, D. J. McConkey, R. Millikan, P. Sweeney, et al.
Treatment with Low-Dose Interferon-{alpha} Restores the Balance between Matrix Metalloproteinase-9 and E-Cadherin Expression in Human Transitional Cell Carcinoma of the Bladder
Clin. Cancer Res., September 1, 2001; 7(9): 2840 - 2853.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society