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Characterization of Corticotropin-Releasing Hormone (CRH) in Human Skin¹

Department of Pathology, Loyola University Medical Center (A.S.), Maywood, Illinois 60153; the Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College (J.E.M.), Albany, New York 12208; Andrus Gerontology Center, University of Southern California (G.E.), Los Angeles, California 90089; and the Department of Medicine, Southern Illinois University (J.W.), Springfield, Illinois 62901

Address all correspondence and requests for reprints to: Andrzej Slominski, M.D., Ph.D., Department of Pathology, Loyola University Medical Center, 2160 South First Avenue, Maywood, Illinois 60153.

We have confirmed the expression of CRH and CRH receptor type 1 genes in human skin, cultured HaCaT keratinocytes, squamous cell carcinoma, and melanoma cells. The size of CRH messenger ribonucleic acid (mRNA), estimated by Northern blot hybridization, was 1.5 kilobases. CRH peptide was identified by reverse phase high pressure liquid chromatography separation in both whole skin and cultured cells. Forskolin and dexamethasone at concentrations of 10 µmol/L stimulated and inhibited, respectively, CRH peptide production in squamous cell carcinoma and melanoma cells, but had no significant effect on the CRH mRNA level. In melanoma cells, stimulation of melanogenesis down-regulated CRH receptor type 1 mRNA expression, but was without effect on CRH mRNA production. We suggest that in human skin the CRH signaling system is both operative and under regulatory control.

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