help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Touraine, P.
Right arrow Articles by Kelly, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Touraine, P.
Right arrow Articles by Kelly, P. A.
The Journal of Clinical Endocrinology & Metabolism Vol. 83, No. 2 667-674
Copyright © 1998 by The Endocrine Society


Original Studies

Increased Expression of Prolactin Receptor Gene Assessed by Quantitative Polymerase Chain Reaction in Human Breast Tumors Versus Normal Breast Tissues

Philippe Touraine, Jean-François Martini, Brigitte Zafrani, Jean-Claude Durand, Françoise Labaille, Catherine Malet, André Nicolas, Christine Trivin, Marie-Catherine Postel-Vinay, Frédérique Kuttenn and Paul A. Kelly

INSERM Unité 344, Faculté Medecine Necker (Ph.T., J-F.M., F.L., M-C.P.V., P.A.K.), 75730 Paris Cedex 15, France; Institut Curie (B.Z., A.N.), 75005 Paris, France; Clinique St. Jean de Dieu (J-C.D.), 75007 Paris, France; Département d’Endocrinologie, Hôpital Necker (C.M., F.K.), Paris, France; Laboratoire d’Explorations Hormonales, Hôpital Necker (C.T.), 75015 Paris, France

Address all correspondence and requests for reprints to: Philippe Touraine, INSERM Unité 344, Faculté Medecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.

The role of PRL in human breast tumorigenesis is not well understood. One of the limitations is the difficulty of accurately measuring PRL receptors (PRLR) in human tissues. We established a quantitative PCR method (Q-PCR) in T-47D human breast cancer cells and applied it to 29 patients, 25 of whom presented with either cancer or fibroadenoma. Four patients underwent a mammoplasty, and normal epithelial cells were cultured before Q-PCR. In T-47D cells, 31 x 106 messenger RNA molecules were detected per microgram of total RNA. In all patients, expression of the PRLR gene was detected, varying from 1500 to 1 x 106 molecules/µg of RNA in normal tissues and from 4500 to 34.7 x 106 molecules/µg of RNA in tumors. PRLR expression was always greater in tumor than in normal contiguous tissue and similar in cultured mammary epithelial cells and normal breast tissues. Estradiol and progesterone receptor-negative tumors expressed low levels of PRLR transcripts, similar to normal breast tissue from menopausal women. Immunocytochemical analysis of PRLR confirmed stronger staining in almost all tumor samples compared with normal tissues. A messenger RNA encoding locally produced human PRL was also identified by RT-PCR in every sample tested. Our results confirm PRLR gene expression in all tissues studied, and moreover, indicate that this expression is increased in human breast tumors vs. normal contiguous tissues.




This article has been cited by other articles:


Home page
Physiol. Rev.Home page
C. Clapp, S. Thebault, M. C. Jeziorski, and G. Martinez De La Escalera
Peptide Hormone Regulation of Angiogenesis
Physiol Rev, October 1, 2009; 89(4): 1177 - 1215.
[Abstract] [Full Text] [PDF]


Home page
J EndocrinolHome page
E. D. Galsgaard, B. B. Rasmussen, C. G. Folkesson, L. M. Rasmussen, M. W. Berchtold, L. Christensen, and S. Panina
Re-evaluation of the prolactin receptor expression in human breast cancer
J. Endocrinol., April 1, 2009; 201(1): 115 - 128.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
F. E. Utama, T. H. Tran, A. Ryder, M. J. LeBaron, A. F. Parlow, and H. Rui
Insensitivity of Human Prolactin Receptors to Nonhuman Prolactins: Relevance for Experimental Modeling of Prolactin Receptor-Expressing Human Cells
Endocrinology, April 1, 2009; 150(4): 1782 - 1790.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
T. M. Piazza, J.-C. Lu, K. C. Carver, and L. A. Schuler
Src Family Kinases Accelerate Prolactin Receptor Internalization, Modulating Trafficking and Signaling in Breast Cancer Cells
Mol. Endocrinol., February 1, 2009; 23(2): 202 - 212.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. L. Bogorad, C. Courtillot, C. Mestayer, S. Bernichtein, L. Harutyunyan, J.-B. Jomain, A. Bachelot, F. Kuttenn, P. A. Kelly, V. Goffin, et al.
Identification of a gain-of-function mutation of the prolactin receptor in women with benign breast tumors
PNAS, September 23, 2008; 105(38): 14533 - 14538.
[Abstract] [Full Text] [PDF]


Home page
Endocr. Rev.Home page
N. Ben-Jonathan, C. R. LaPensee, and E. W. LaPensee
What Can We Learn from Rodents about Prolactin in Humans?
Endocr. Rev., February 1, 2008; 29(1): 1 - 41.
[Abstract] [Full Text] [PDF]


Home page
J EndocrinolHome page
C. Manhes, C. Kayser, P. Bertheau, B. Kelder, J. J Kopchick, P. A Kelly, P. Touraine, and V. Goffin
Local over-expression of prolactin in differentiating mouse mammary gland induces functional defects and benign lesions, but no carcinoma.
J. Endocrinol., August 1, 2006; 190(2): 271 - 285.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
A. M. Qazi, C.-H. Tsai-Morris, and M. L. Dufau
Ligand-Independent Homo- and Heterodimerization of Human Prolactin Receptor Variants: Inhibitory Action of the Short Forms by Heterodimerization
Mol. Endocrinol., August 1, 2006; 20(8): 1912 - 1923.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
A. Vaclavicek, K. Hemminki, C. R. Bartram, K. Wagner, B. Wappenschmidt, A. Meindl, R. K. Schmutzler, R. Klaes, M. Untch, B. Burwinkel, et al.
Association of Prolactin and Its Receptor Gene Regions with Familial Breast Cancer
J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1513 - 1519.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
L. M. Arendt, T. A. Rose-Hellekant, E. P. Sandgren, and L. A. Schuler
Prolactin Potentiates Transforming Growth Factor {alpha} Induction of Mammary Neoplasia in Transgenic Mice
Am. J. Pathol., April 1, 2006; 168(4): 1365 - 1374.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
S. S. Tworoger, P. Sluss, and S. E. Hankinson
Association between Plasma Prolactin Concentrations and Risk of Breast Cancer among Predominately Premenopausal Women
Cancer Res., February 15, 2006; 66(4): 2476 - 2482.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
Z. Nouhi, N. Chughtai, S. Hartley, E. Cocolakis, J.-J. Lebrun, and S. Ali
Defining the Role of Prolactin as an Invasion Suppressor Hormone in Breast Cancer Cells
Cancer Res., February 1, 2006; 66(3): 1824 - 1832.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J.-C. Lu, T. M. Piazza, and L. A. Schuler
Proteasomes Mediate Prolactin-induced Receptor Down-regulation and Fragment Generation in Breast Cancer Cells
J. Biol. Chem., October 7, 2005; 280(40): 33909 - 33916.
[Abstract] [Full Text] [PDF]


Home page
Endocr. Rev.Home page
V. Goffin, S. Bernichtein, P. Touraine, and P. A. Kelly
Development and Potential Clinical Uses of Human Prolactin Receptor Antagonists
Endocr. Rev., May 1, 2005; 26(3): 400 - 422.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
F. Arturi, E. Ferretti, I. Presta, T. Mattei, A. Scipioni, D. Scarpelli, R. Bruno, L. Lacroix, E. Tosi, A. Gulino, et al.
Regulation of Iodide Uptake and Sodium/Iodide Symporter Expression in the MCF-7 Human Breast Cancer Cell Line
J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2321 - 2326.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. J. Waters and B. L. Conway-Campbell
The oncogenic potential of autocrine human growth hormone in breast cancer
PNAS, October 19, 2004; 101(42): 14992 - 14993.
[Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
D. Piwnica, P. Touraine, I. Struman, S. Tabruyn, G. Bolbach, C. Clapp, J. A. Martial, P. A. Kelly, and V. Goffin
Cathepsin D Processes Human Prolactin into Multiple 16K-Like N-Terminal Fragments: Study of Their Antiangiogenic Properties and Physiological Relevance
Mol. Endocrinol., October 1, 2004; 18(10): 2522 - 2542.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. Meng, C.-H. Tsai-Morris, and M. L. Dufau
Human Prolactin Receptor Variants in Breast Cancer: Low Ratio of Short Forms to the Long-Form Human Prolactin Receptor Associated with Mammary Carcinoma
Cancer Res., August 15, 2004; 64(16): 5677 - 5682.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
M. D. Schroeder, J. L. Brockman, A. M. Walker, and L. A. Schuler
Inhibition of Prolactin (PRL)-Induced Proliferative Signals in Breast Cancer Cells by a Molecular Mimic of Phosphorylated PRL, S179D-PRL
Endocrinology, December 1, 2003; 144(12): 5300 - 5307.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
J. J. Acosta, R. M. Munoz, L. Gonzalez, A. Subtil-Rodriguez, M. A. Dominguez-Caceres, J. M. Garcia-Martinez, A. Calcabrini, I. Lazaro-Trueba, and J. Martin-Perez
Src Mediates Prolactin-Dependent Proliferation of T47D and MCF7 Cells via the Activation of Focal Adhesion Kinase/Erk1/2 and Phosphatidylinositol 3-Kinase Pathways
Mol. Endocrinol., November 1, 2003; 17(11): 2268 - 2282.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Bernichtein, C. Kayser, K. Dillner, S. Moulin, J. J. Kopchick, J. A. Martial, G. Norstedt, O. Isaksson, P. A. Kelly, and V. Goffin
Development of Pure Prolactin Receptor Antagonists
J. Biol. Chem., September 19, 2003; 278(38): 35988 - 35999.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
E. Saunier, F. Dif, P. A. Kelly, and M. Edery
Targeted Expression of the Dominant-Negative Prolactin Receptor in the Mammary Gland of Transgenic Mice Results in Impaired Lactation
Endocrinology, June 1, 2003; 144(6): 2669 - 2675.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
M. Zinger, M. McFarland, and N. Ben-Jonathan
Prolactin Expression and Secretion by Human Breast Glandular and Adipose Tissue Explants
J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 689 - 696.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
B. M. Jacobsen, J. K. Richer, S. A. Schittone, and K. B. Horwitz
New Human Breast Cancer Cells to Study Progesterone Receptor Isoform Ratio Effects and Ligand-independent Gene Regulation
J. Biol. Chem., July 26, 2002; 277(31): 27793 - 27800.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
J. L. Brockman, M. D. Schroeder, and L. A. Schuler
PRL Activates the Cyclin D1 Promoter Via the Jak2/Stat Pathway
Mol. Endocrinol., April 1, 2002; 16(4): 774 - 784.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
M. D. Schroeder, J. Symowicz, and L. A. Schuler
PRL Modulates Cell Cycle Regulators in Mammary Tumor Epithelial Cells
Mol. Endocrinol., January 1, 2002; 16(1): 45 - 57.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
A. Glasow, L.-C. Horn, S. E. Taymans, C. A. Stratakis, P. A. Kelly, U. Kohler, J. Gillespie, B. K. Vonderhaar, and S. R. Bornstein
Mutational Analysis of the PRL Receptor Gene in Human Breast Tumors with Differential PRL Receptor Protein Expression
J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3826 - 3832.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
M. Gebre-Medhin, L.-G. Kindblom, H. Wennbo, J. Tornell, and J. M. Meis-Kindblom
Growth Hormone Receptor Is Expressed in Human Breast Cancer
Am. J. Pathol., April 1, 2001; 158(4): 1217 - 1222.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
J.-Y. Cho, R. Léveillé, R. Kao, B. Rousset, A. F. Parlow, W. E. Burak Jr., E. L. Mazzaferri, and S. M. Jhiang
Hormonal Regulation of Radioiodide Uptake Activity and Na+/I- Symporter Expression in Mammary Glands
J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2936 - 2943.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
D. Melck, L. De Petrocellis, P. Orlando, T. Bisogno, C. Laezza, M. Bifulco, and V. Di Marzo
Suppression of Nerve Growth Factor Trk Receptors and Prolactin Receptors by Endocannabinoids Leads to Inhibition of Human Breast and Prostate Cancer Cell Proliferation
Endocrinology, January 1, 2000; 141(1): 118 - 126.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
B. Tsunekawa, M. Wada, M. Ikeda, H. Uchida, N. Naito, and M. Honjo
The 20-Kilodalton (kDa) Human Growth Hormone (hGH) Differs from the 22-kDa hGH in the Effect on the Human Prolactin Receptor
Endocrinology, September 1, 1999; 140(9): 3909 - 3918.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society