This Article Full Text Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, J.-C. Articles by Dawood, M. Y. Search for Related Content PubMed PubMed Citation Articles by Huang, J.-C. Articles by Dawood, M. Y.

Interleukin-1ß Induces Cyclooxygenase-2 Gene Expression in Cultured Endometrial Stromal Cells

Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Reproductive Endocrinology, and the Department of Internal Medicine, Division of Hematology (K.K.W.), University of Texas Health Science Center, Houston, Texas 77030

Address all correspondence and requests for reprints to: Jaou-Chen Huang, M.D., Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School, 6431 Fannin, MSB 3.036, Houston, Texas 77030. E-mail: jhuang{at}obg.med.uth.tmc.edu

Increasing evidence indicates that PGs may play an obligatory role in blastocyst implantation. Cyclooxygenase (also known as PGH synthase) isozymes 1 and 2 catalyze the rate-limiting steps in the biosynthesis of PGs. The ubiquitous cyclooxygenase-1 (COX-1) subserves housekeeping functions, whereas the inducible cyclooxygenase-2 (COX-2) is expressed by limited cell types and tightly controlled. Here we report the induction of COX-2 gene expression by interleukin-1ß (IL-1ß) in cultured human endometrial stromal cells.

COX-2 activity was induced by IL-1ß (1 ng/mL); conversion of exogenous arachidonic acid to PGF₂ increased from 2.6 ± 0.6 ng/well (mean ± SEM; n = 6) to 22.2 ± 5.6 ng, but was completely blocked (2.8 ± 0.7 ng/well) by NS-398, a specific COX-2 inhibitor. Undetectable in quiescent stromal cells, messenger ribonucleic acid for COX-2 was induced 30 min after IL-1ß treatment, reached a maximum at 4 h, and decreased after 15 h. Protein synthesis was not required for induction of the COX-2 gene, as it was blocked by actinomycin D but not by cycloheximide. The 70-kDa COX-2 protein was not detected in quiescent cells, became detectable 6 h after IL-1ß treatment, and remained detectable even after 15 h. IL-1ß (0.1–100 ng/mL) increased the luciferase activity in promoterless luciferase reporter containing the 900-bp 5'-flanking sequence (-891 to +9) of the COX-2 gene in a dose-dependent manner, with an ED₅₀ of 0.1–1 ng/mL.

This article has been cited by other articles:

				A.A Fouladi-Nashta, L Mohamet, J.K Heath, and S.J Kimber Interleukin 1 Signaling Is Regulated by Leukemia Inhibitory Factor (LIF) and Is Aberrant in Lif-/- Mouse Uterus Biol Reprod, July 1, 2008; 79(1): 142 - 153. [Abstract] [Full Text] [PDF]

				M. Ulukus, H. Cakmak, and A. Arici The Role of Endometrium in Endometriosis Reproductive Sciences, October 1, 2006; 13(7): 467 - 476. [Abstract] [PDF]

				P. Chapdelaine, J. Kang, S. Boucher-Kovalik, N. Caron, J. P. Tremblay, and M. A. Fortier Decidualization and maintenance of a functional prostaglandin system in human endometrial cell lines following transformation with SV40 large T antigen Mol. Hum. Reprod., May 1, 2006; 12(5): 309 - 319. [Abstract] [Full Text] [PDF]

				A W Horne, E-N Lalani, R A Margara, and J O White The effects of sex steroid hormones and interleukin-1-beta on MUC1 expression in endometrial epithelial cell lines. Reproduction, April 1, 2006; 131(4): 733 - 742. [Abstract] [Full Text] [PDF]

				Z. Strakova, P. Mavrogianis, X. Meng, J. M. Hastings, K. S. Jackson, P. Cameo, A. Brudney, O. Knight, and A. T. Fazleabas In Vivo Infusion of Interleukin-1{beta} and Chorionic Gonadotropin Induces Endometrial Changes that Mimic Early Pregnancy Events in the Baboon Endocrinology, September 1, 2005; 146(9): 4097 - 4104. [Abstract] [Full Text] [PDF]

				J. Kang, A. Akoum, P. Chapdelaine, P. Laberge, P. E. Poubelle, and M. A. Fortier Independent regulation of prostaglandins and monocyte chemoattractant protein-1 by interleukin-1{beta} and hCG in human endometrial cells Hum. Reprod., November 1, 2004; 19(11): 2465 - 2473. [Abstract] [Full Text] [PDF]

				M. Almagor, A. Hazav, and H. Yaffe The levels of C-reactive protein in women treated by IVF Hum. Reprod., January 1, 2004; 19(1): 104 - 106. [Abstract] [Full Text] [PDF]

				J. W. Ross, J. R. Malayer, J. W. Ritchey, and R. D. Geisert Characterization of the Interleukin-1{beta} System During Porcine Trophoblastic Elongation and Early Placental Attachment Biol Reprod, October 1, 2003; 69(4): 1251 - 1259. [Abstract] [Full Text] [PDF]

				V. Shim, M. L. Gauthier, D. Sudilovsky, K. Mantei, K. L. Chew, D. H. Moore, I. Cha, T. D. Tlsty, and L. J. Esserman Cyclooxygenase-2 Expression Is Related to Nuclear Grade in Ductal Carcinoma in Situ and Is Increased in Its Normal Adjacent Epithelium Cancer Res., May 15, 2003; 63(10): 2347 - 2350. [Abstract] [Full Text] [PDF]

				O. Yoshino, Y. Osuga, Y. Hirota, K. Koga, T. Hirata, T. Yano, T. Ayabe, O. Tsutsumi, and Y. Taketani Endometrial Stromal Cells Undergoing Decidualization Down-Regulate Their Properties to Produce Proinflammatory Cytokines in Response to Interleukin-1{beta} via Reduced p38 Mitogen-Activated Protein Kinase Phosphorylation J. Clin. Endocrinol. Metab., May 1, 2003; 88(5): 2236 - 2241. [Abstract] [Full Text] [PDF]

				J.-G. Cheng and C. L. Stewart Loss of Cyclooxygenase-2 Retards Decidual Growth but Does Not Inhibit Embryo Implantation or Development to Term Biol Reprod, February 1, 2003; 68(2): 401 - 404. [Abstract] [Full Text] [PDF]

				F. Arbab, J. Goldsby, N. Matijevic-Aleksic, G. Huang, K.-H. Ruan, and J.-C. Huang Prostacyclin is an autocrine regulator in the contraction of oviductal smooth muscle Hum. Reprod., December 1, 2002; 17(12): 3053 - 3059. [Abstract] [Full Text] [PDF]

				J.-C. Huang, F. Arbab, K. J. Tumbusch, J. S. Goldsby, N. Matijevic-Aleksic, and K. K. Wu Human Fallopian Tubes Express Prostacyclin (PGI) Synthase and Cyclooxygenases and Synthesize Abundant PGI J. Clin. Endocrinol. Metab., September 1, 2002; 87(9): 4361 - 4368. [Abstract] [Full Text] [PDF]

				M. Tamura, S. Sebastian, S. Yang, B. Gurates, K. Ferrer, H. Sasano, K. Okamura, and S. E. Bulun Up-regulation of Cyclooxygenase-2 Expression and Prostaglandin Synthesis in Endometrial Stromal Cells by Malignant Endometrial Epithelial Cells. A PARACRINE EFFECT MEDIATED BY PROSTAGLANDIN E2 AND NUCLEAR FACTOR-kappa B J. Biol. Chem., July 12, 2002; 277(29): 26208 - 26216. [Abstract] [Full Text] [PDF]

				M. Tamura, S. Sebastian, S. Yang, B. Gurates, Z. Fang, and S. E. Bulun Interleukin-1{beta} Elevates Cyclooxygenase-2 Protein Level and Enzyme Activity via Increasing Its mRNA Stability in Human Endometrial Stromal Cells: An Effect Mediated by Extracellularly Regulated Kinases 1 and 2 J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3263 - 3273. [Abstract] [Full Text] [PDF]

				E. R. Norwitz, D. J. Schust, and S. J. Fisher Implantation and the Survival of Early Pregnancy N. Engl. J. Med., November 8, 2001; 345(19): 1400 - 1408. [Full Text] [PDF]

				Z. Strakova, S. Srisuparp, and A. T. Fazleabas Interleukin-1{beta} Induces the Expression of Insulin-Like Growth Factor Binding Protein-1 during Decidualization in the Primate Endocrinology, December 1, 2000; 141(12): 4664 - 4670. [Abstract] [Full Text] [PDF]