help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kumar, S.
Right arrow Articles by Bagchi, I. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumar, S.
Right arrow Articles by Bagchi, I. C.
The Journal of Clinical Endocrinology & Metabolism Vol. 83, No. 12 4443-4450
Copyright © 1998 by The Endocrine Society

Original Studies

Progesterone Induces Calcitonin Gene Expression in Human Endometrium within the Putative Window of Implantation1

Sushma Kumar, Li-Ji Zhu, Mary Polihronis, Sharon T. Cameron, David T. Baird, Frederick Schatz, Anuradha Dua, Yu-Kang Ying, Milan K. Bagchi and Indrani C. Bagchi

Population Council and The Rockefeller University, New York, New York 10021; the Department of Obstetrics and Gynecology, Nassau County Medical Center, (S.K., A.D., Y.-K.Y.), New York, New York 11554; the Department of Obstetrics and Gynecology, Center for Reproductive Biology, University of Edinburgh (S.T.C., D.T.B.), Edinburgh, United Kingdom; and the Department of Obstetrics and Gynecology, New York University Medical School (F.S.), New York, New York 10016

Address all correspondence and requests for reprints to: Dr. Indrani C. Bagchi, The Population Council, 1230 York Avenue, New York, New York 10021. E-mail: indrani{at}popcbr.rockefeller.edu

The human endometrium acquires the ability to implant the developing embryo within a specific time window that is thought to open between days 19–24 of the secretory phase of the menstrual cycle. During this period the endometrium undergoes pronounced structural and functional changes induced by the ovarian steroids, estrogen and progesterone, that prepare it to be receptive to invasion by the embryo. The identification of reliable biochemical markers to assess this critical receptive phase in the context of the natural cycle remains one of the major challenges in the study of human reproduction. Our previous studies in a rat model system demonstrated that the expression of calcitonin, a peptide hormone involved in calcium homeostasis, is transiently induced by progesterone in the glandular epithelium at the onset of implantation. Attenuation of calcitonin synthesis in the uterus during the preimplantation phase by administration of calcitonin antisense oligodeoxynucleotides severely impairs implantation of rat embryos, suggesting that this peptide hormone plays a critical role in uterine receptivity. To investigate whether calcitonin is also expressed in the human endometrium during implantation, we monitored the spatio-temporal expression of calcitonin on various days of the menstrual cycle. Our studies employing RT-PCR showed that calcitonin messenger ribonucleic acid is expressed in human endometrium during the postovulatory midsecretory phase (days 17–25) of the menstrual cycle, with maximal expression occurring between days 19–21. Very little calcitonin expression was detected in the endometrium in either the preovulatory proliferative (days 5–14) or the late secretory (days 26–28) phase. In situ hybridization and immunocytochemical analyses localized the calcitonin expression predominantly in the glandular epithelial cells of the endometrium. Our studies further showed that calcitonin expression in the human endometrium is under progesterone regulation. Treatment of women with an antiprogestin, mifepristone (RU-486), drastically reduced calcitonin expression in the endometrium. Collectively, these findings reveal that progesterone-induced expression of calcitonin in the secretory endometrium temporally coincides with the putative window of implantation in the human.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
S. M Krzysik-Walker, O. M Ocon-Grove, S. B Maddineni, G. L Hendricks III, and R. Ramachandran
Identification of Calcitonin Expression in the Chicken Ovary: Influence of Follicular Maturation and Ovarian Steroids
Biol Reprod, October 1, 2007; 77(4): 626 - 635.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
H. Achache and A. Revel
Endometrial receptivity markers, the journey to successful embryo implantation
Hum. Reprod. Update, November 1, 2006; 12(6): 731 - 746.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
J-W Jeong, K Y Lee, J P Lydon, and F J DeMayo
Steroid hormone regulation of Clca3 expression in the murine uterus.
J. Endocrinol., June 1, 2006; 189(3): 473 - 484.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Q. Li, M. K. Bagchi, and I. C. Bagchi
Identification of a Signaling Pathway Involving Progesterone Receptor, Calcitonin, and Tissue Tranglutaminase in Ishikawa Endometrial Cells
Endocrinology, May 1, 2006; 147(5): 2147 - 2154.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
H. N. Jabbour, R. W. Kelly, H. M. Fraser, and H. O. D. Critchley
Endocrine Regulation of Menstruation
Endocr. Rev., February 1, 2006; 27(1): 17 - 46.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J.-W. Jeong, K. Y. Lee, I. Kwak, L. D. White, S. G. Hilsenbeck, J. P. Lydon, and F. J. DeMayo
Identification of Murine Uterine Genes Regulated in a Ligand-Dependent Manner by the Progesterone Receptor
Endocrinology, August 1, 2005; 146(8): 3490 - 3505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. R. McDonald, N. J. Fudge, J. P. Woodrow, J. K. Friel, A. O. Hoff, R. F. Gagel, and C. S. Kovacs
Ablation of calcitonin/calcitonin gene-related peptide-{alpha} impairs fetal magnesium but not calcium homeostasis
Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E218 - E226.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
H. S. Taylor
Endometrial Cells Derived From Donor Stem Cells in Bone Marrow Transplant Recipients
JAMA, July 7, 2004; 292(1): 81 - 85.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
K. Gemzell-Danielsson and L. Marions
Mechanisms of action of mifepristone and levonorgestrel when used for emergency contraception
Hum. Reprod. Update, July 1, 2004; 10(4): 341 - 348.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R.D. Catalano, A. Yanaihara, A.L. Evans, D. Rocha, A. Prentice, S. Saidi, C.G. Print, D.S. Charnock-Jones, A.M. Sharkey, and S.K. Smith
The effect of RU486 on the gene expression profile in an endometrial explant model
Mol. Hum. Reprod., August 1, 2003; 9(8): 465 - 473.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Kumar, A. Brudney, Y.-P. Cheon, A. T. Fazleabas, and I. C. Bagchi
Progesterone Induces Calcitonin Expression in the Baboon Endometrium Within the Window of Uterine Receptivity
Biol Reprod, April 1, 2003; 68(4): 1318 - 1323.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
N. Chegini, C. Ma, X.M. Tang, and R.S. Williams
Effects of GnRH analogues, `add-back' steroid therapy, antiestrogen and antiprogestins on leiomyoma and myometrial smooth muscle cell growth and transforming growth factor-{beta} expression
Mol. Hum. Reprod., December 1, 2002; 8(12): 1071 - 1078.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
Y.-P. Cheon, Q. Li, X. Xu, F. J. DeMayo, I. C. Bagchi, and M. K. Bagchi
A Genomic Approach to Identify Novel Progesterone Receptor Regulated Pathways in the Uterus during Implantation
Mol. Endocrinol., December 1, 2002; 16(12): 2853 - 2871.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Li, J. Wang, D. R. Armant, M. K. Bagchi, and I. C. Bagchi
Calcitonin Down-regulates E-cadherin Expression in Rodent Uterine Epithelium during Implantation
J. Biol. Chem., November 22, 2002; 277(48): 46447 - 46455.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Reese, S. K. Das, B. C. Paria, H. Lim, H. Song, H. Matsumoto, K. L. Knudtson, R. N. DuBois, and S. K. Dey
Global Gene Expression Analysis to Identify Molecular Markers of Uterine Receptivity and Embryo Implantation
J. Biol. Chem., November 16, 2001; 276(47): 44137 - 44145.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
I. C. BAGCHI, Q. LI, and Y. P. CHEON
Role of Steroid Hormone-Regulated Genes in Implantation
Ann. N.Y. Acad. Sci., September 1, 2001; 943(1): 68 - 76.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
H.-Y. Li, S.-P. Chang, C.-C. Yuan, H.-T. Chao, H.-T. Ng, and Y.-J. Sung
Nitric oxide induces extensive apoptosis in endometrial epithelial cells in the presence of progesterone: involvement of mitogen-activated protein kinase pathways
Mol. Hum. Reprod., August 1, 2001; 7(8): 755 - 763.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Pavelock, K. M. Braas, L'H. Ouafik, G. Osol, and V. May
Differential Expression and Regulation of the Vascular Endothelial Growth Factor Receptors Neuropilin-1 and Neuropilin-2 in Rat Uterus
Endocrinology, February 1, 2001; 142(2): 613 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
Y. Gui, J. Zhang, L. Yuan, and B. A. Lessey
Regulation of HOXA-10 and its expression in normal and abnormal endometrium
Mol. Hum. Reprod., September 1, 1999; 5(9): 866 - 873.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society