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Lactate Metabolism of Subcutaneous Adipose Tissue Studied by Open Flow Microperfusion

Department of Internal Medicine and Diabetes and Metabolism, Karl Franzens University Graz (M.E., G.S., A.W., G.A.B., P.W., T.R.P.), A-8036 Graz; the Department of Biophysic, Institute of Electro- and Biomedical Engineering, University of Technology Graz (M.E., L.S., Z.T., P.W.), A-8010 Graz; and the Department of Internal Medicine, Krankenhaus der Barmherzigen Brüder, Teaching Hospital, Karl Franzens University Graz (F.S.), 8020 Graz, Austria

Address all correspondence and requests for reprints to: Thomas Pieber, M.D., Department of Internal Medicine, Diabetes and Metabolism, Karl Franzens University Graz, Auenbruggerplatz 15, A-8036 Graz, Austria. E-mail: thomas.pieber{at}kfunigraz.ac.at

Open flow microperfusion and a novel calibration technique (ionic reference technique) were evaluated for the frequent measurement of the absolute lactate concentration in sc adipose tissue. Furthermore, the influence of the plasma insulin concentration on the lactate concentration of sc adipose tissue was investigated during hyperglycemia. Sixteen lean healthy young men participated in the studies. In the postabsorbtive state the mean sc lactate concentrations were 1.29 and 1.36 mmol/L for the ionic reference technique and the no net flux protocol, respectively (not significant, P > 0.05). The simultaneously measured arterialized plasma lactate concentration was significantly lower at 0.77 mmol/L (P < 0.05). Both the sc lactate concentration (1.8 ± 0.33 mmol/L) and the plasma lactate concentration (0.96 ± 0.03 mmol/L) were significantly elevated during a hyperinsulinemic euglycemic clamp experiment. During a hyperglycemic clamp experiment the sc lactate concentration reached a significantly elevated plateau (2.15 ± 0.27 mmol/L) that was not influenced by the increasing plasma insulin concentration. It is concluded that 1) open flow microperfusion combined with the ionic reference technique enables frequent measurement of the sc lactate concentration; 2) sc adipose tissue is a significant source of lactate release in the postabsorbtive state as well as during hyperinsulinemic clamp conditions; and 3) insulin concentrations greater than 180 pmol/L have no further influence on adipocyte stimulation of sc adipose tissue with respect to lactate release.

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