help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints, Permissions and Rights
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Machelon, V.
Right arrow Articles by Tesarik, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Machelon, V.
Right arrow Articles by Tesarik, J.
The Journal of Clinical Endocrinology & Metabolism Vol. 83, No. 1 263-269
Copyright © 1998 by The Endocrine Society

Original Studies

Nongenomic Effects of Androstenedione on Human Granulosa Luteinizing Cells

Véronique Machelon, Françoise Nomé and Jan Tesarik

INSERM, U-355, Institut Paris-Sud sur les Cytokines (V.M., F.N.), 92140 Clamart; Laboratoire d’Eylau (J.T.), 75116 Paris, France

Address all correspondence and requests for reprints to: Dr. Véronique Machelon, INSERM, U-355, Maturation Gamétique et Fécondation, 32 rue des Carnets, 92140 Clamart, France.

This study examines rapid (5–60 s) effects of androgens on the cytosolic free Ca2+ concentration ([Ca2+]i) in human granulosa luteinizing cells. Cells were obtained from human preovulatory follicles, and [Ca2+]i was measured with the use of the Ca2+-responsive fluorescent dye fluo-3. Molar concentrations between 100 pmol/L and 1 µmol/L androstenedione increased [Ca2+]i within 5 s after addition to cells. This [Ca2+]i increase resulted from both Ca2+ influx, as shown by the effects of ethyleneglycol-bis-(ß-aminoethyl ether)-N,N,N',N'-tetraacetic acid and the voltage-dependent Ca2+ channel blocker verapamil, and Ca2+ mobilization from the endoplasmic reticulum, as shown by the effects of thapsigargin. Treatment with pertussis toxin and U-73,122, a specific inhibitor of phospholipase C, abolished the effects of androstenedione on [Ca2+]i. Flutamide, a nuclear androgen receptor antagonist, did not block the increase in [Ca2+]i induced by androstenedione. Testosterone (100 pmol/L to 1 µmol/L) had no effect. This is the first report showing that androstenedione increases [Ca2+]i in granulosa cells. These data provide evidence for the presence in granulosa cells of a novel, short term mechanism of androstenedione action involving voltage-dependent Ca2+ channels in the plasma membrane and phospholipase C activation via a pertussis toxin-sensitive G protein.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
D. Liu, M. Iruthayanathan, L. L. Homan, Y. Wang, L. Yang, Y. Wang, and J. S. Dillon
Dehydroepiandrosterone Stimulates Endothelial Proliferation and Angiogenesis through Extracellular Signal-Regulated Kinase 1/2-Mediated Mechanisms
Endocrinology, March 1, 2008; 149(3): 889 - 898.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Liu, H. Si, K. A. Reynolds, W. Zhen, Z. Jia, and J. S. Dillon
Dehydroepiandrosterone Protects Vascular Endothelial Cells against Apoptosis through a G{alpha}i Protein-Dependent Activation of Phosphatidylinositol 3-Kinase/Akt and Regulation of Antiapoptotic Bcl-2 Expression
Endocrinology, July 1, 2007; 148(7): 3068 - 3076.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Y.-H. Sun, X. Gao, Y.-J. Tang, C.-L. Xu, and L.-H. Wang
Androgens Induce Increases in Intracellular Calcium Via a G Protein-Coupled Receptor in LNCaP Prostate Cancer Cells
J Androl, September 1, 2006; 27(5): 671 - 678.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
Y. Wu, W. G Foster, and E. V Younglai
Rapid effects of pesticides on human granulosa-lutein cells
Reproduction, February 1, 2006; 131(2): 299 - 310.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. Casais, S. M. Delgado, Z. Sosa, and A. M. Rastrilla
Involvement of the coeliac ganglion in the luteotrophic effect of androstenedione in late pregnant rats
Reproduction, February 1, 2006; 131(2): 361 - 368.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E.V. Younglai, Y.J. Wu, T.K. Kwan, and C.-Y. Kwan
Non-genomic action of estradiol and progesterone on cytosolic calcium concentrations in primary cultures of human granulosa-lutein cells
Hum. Reprod., September 1, 2005; 20(9): 2383 - 2390.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
D. Platano, M. C. Magli, A. P. Ferraretti, L. Gianaroli, and G. Aicardi
L- and T-Type Voltage-Gated Ca2+ Channels in Human Granulosa Cells: Functional Characterization and Cholinergic Regulation
J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2192 - 2197.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. M. Braun and P. Thomas
Biochemical Characterization of a Membrane Androgen Receptor in the Ovary of the Atlantic Croaker (Micropogonias undulatus)
Biol Reprod, July 1, 2004; 71(1): 146 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. M. Braun and P. Thomas
Androgens Inhibit Estradiol-17{beta} Synthesis in Atlantic Croaker (Micropogonias undulatus) Ovaries by a Nongenomic Mechanism Initiated at the Cell Surface
Biol Reprod, November 1, 2003; 69(5): 1642 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. M. LOSEL, E. FALKENSTEIN, M. FEURING, A. SCHULTZ, H.-C. TILLMANN, K. ROSSOL-HASEROTH, and M. WEHLING
Nongenomic Steroid Action: Controversies, Questions, and Answers
Physiol Rev, July 1, 2003; 83(3): 965 - 1016.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. A. Goyeneche, V. Calvo, G. Gibori, and C. M. Telleria
Androstenedione Interferes in Luteal Regression by Inhibiting Apoptosis and Stimulating Progesterone Production
Biol Reprod, May 1, 2002; 66(5): 1540 - 1547.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
S. F. Palter, A. B. Tavares, A. Hourvitz, J. D. Veldhuis, and E. Y. Adashi
Are Estrogens of Import to Primate/Human Ovarian Folliculogenesis?
Endocr. Rev., June 1, 2001; 22(3): 389 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
E. Falkenstein, H.-C. Tillmann, M. Christ, M. Feuring, and M. Wehling
Multiple Actions of Steroid Hormones---A Focus on Rapid, Nongenomic Effects
Pharmacol. Rev., December 1, 2000; 52(4): 513 - 556.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F.M. Lyng, G.R. Jones, and F.F.G. Rommerts
Rapid Androgen Actions on Calcium Signaling in Rat Sertoli Cells and Two Human Prostatic Cell Lines: Similar Biphasic Responses Between 1 Picomolar and 100 Nanomolar Concentrations
Biol Reprod, September 1, 2000; 63(3): 736 - 747.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
Y. Morita and J. L. Tilly
Segregation of Retinoic Acid Effects on Fetal Ovarian Germ Cell Mitosis Versus Apoptosis by Requirement for New Macromolecular Synthesis
Endocrinology, June 1, 1999; 140(6): 2696 - 2703.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
R. Varghese, C. K. C. Wong, H. Deol, G. F. Wagner, and G. E. DiMattia
Comparative Analysis of Mammalian Stanniocalcin Genes
Endocrinology, November 1, 1998; 139(11): 4714 - 4725.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society